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Electrochemical detection of Salmonella using gold nanoparticles

AuthorsAfonso, André S.; Pérez-López, Briza ; Maltez, Marisa ; Merkoçi, Arben
Electrochemical detection
Gold nanoparticles
Issue Date2013
CitationBiosensors and Bioelectronics 40(1): 121-126 (2013)
AbstractA disposable immunosensor for Salmonella enterica subsp. enterica serovar Typhimurium LT2 (S) detection using a magneto-immunoassay and gold nanoparticles (AuNPs) as label for electrochemical detection is developed. The immunosensor is based on the use of a screen-printed carbon electrode (SPCE) that incorporates a permanent magnet underneath. Salmonella containing samples (i.e. skimmed milk) have been tested by using anti-Salmonella magnetic beads (MBs-pSAb) as capture phase and sandwiching afterwards with AuNPs modified antibodies (sSAb-AuNPs) detected using differential pulse voltammetry (DPV). A detection limit of 143 cellsmL -1 and a linear range from 10 3 to 10 6 cellsmL -1 of Salmonella was obtained, with a coefficient of variation of about 2.4%. Recoveries of the sensor by spiking skimmed milk with different quantities of Salmonella of about 83% and 94% for 1.5×10 3 and 1.5×10 5 cellsmL -1 were obtained, respectively. This AuNPs detection technology combined with magnetic field application reports a limit of detection lower than the conventional commercial method carried out for comparison purposes in skimmed milk samples. © 2012 Elsevier B.V.
Identifiersdoi: 10.1016/j.bios.2012.06.054
issn: 0956-5663
e-issn: 1873-4235
Appears in Collections:(CIN2) Artículos
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