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http://hdl.handle.net/10261/99277
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dc.contributor.author | Villacieros, Marta | - |
dc.contributor.author | Whelan, Clare | - |
dc.contributor.author | Mackova, Martina | - |
dc.contributor.author | Molgaard, Jesper | - |
dc.contributor.author | Sánchez-Contreras, María | - |
dc.contributor.author | Lloret, Javier | - |
dc.contributor.author | Aguirre de Cárcer, Daniel | - |
dc.contributor.author | Oruezábal, Roke I. | - |
dc.contributor.author | Bolaños, Luis | - |
dc.contributor.author | Macek, Thomas | - |
dc.contributor.author | Karlson, Ulrich | - |
dc.contributor.author | Dowling, David N. | - |
dc.contributor.author | Martín, Marta | - |
dc.contributor.author | Rivilla, Rafael | - |
dc.date.accessioned | 2014-07-02T09:11:10Z | - |
dc.date.available | 2014-07-02T09:11:10Z | - |
dc.date.issued | 2014-07-02 | - |
dc.identifier.uri | http://hdl.handle.net/10261/99277 | - |
dc.description.abstract | Rhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a derivative of F113 expressing polychlorinated biphenyl (PCB) biodegradation genes (F113pcb) has been reported previously, this strain shows a low level of bph gene expression, limiting its rhizoremediation potential. Here, a high-level expression system was designed from rhizobial nod gene regulatory relays. Nod promoters were tested in strain F113 by using _-galactosidase transcriptional fusions. This analysis showed that nodbox 4 from Sinorhizobium meliloti has a high level of expression in F113 that is dependent on an intact nodD1 gene. A transcriptional fusion of a nodbox cassette containing the nodD1 gene and nodbox 4 fused to a gfp gene was expressed in the alfalfa rhizosphere. The bph operon from Burkholderia sp. strain LB400 was cloned under the control of the nodbox cassette and was inserted as a single copy into the genome of F113, generating strain F113L::1180. This new genetically modified strain has a high level of BphC activity and grows on biphenyl as a sole carbon and energy source at a growth rate that is more than three times higher than that of F113pcb. Degradation of PCBs 3, 4, 5, 17, and 25 was also much faster in F113L::1180 than in F113pcb. Finally, the modified strain cometabolized PCB congeners present in Delor103 better than strain LB400, the donor of the bph genes used. | es_ES |
dc.description.sponsorship | Ministerio de Ciencia y Tecnología; European Commission grants BIO4-CT97-2227 and QLK3-CT-2001-00101 | es_ES |
dc.language.iso | eng | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.title | Polychlorinated Biphenyl Rhizoremediation by Pseudomonas fluorescens F113 Derivatives, Using a Sinorhizobium meliloti nod System To Drive bph Gene Expression | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1128/AEM.71.5.2687–2694.2005 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.csic | No | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
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