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T-RFLP detection of bacterial populations potentially resident in the abomasum of lactating sheep

AutorCastro Carrera, Tamara ; Toral, Pablo G. ; Hervás, Gonzalo ; Frutos, Pilar ; Belenguer, Álvaro
Fecha de publicaciónjun-2014
EditorRowett Institute of Nutrition and Health
CitaciónGut microbiology: from sequence to function 73 (P052) (2014)
ResumenPrevious works in ruminants have reported the existence of microorganisms resident in the abomasum, which could be present at noticeable concentrations despite the low pH and short transit time. These populations may not match bacteria established in the rumen, although there is little information about it. In this study, the terminal restriction fragment length polymorphism (T-RFLP) technique was employed to detect bacteria colonizing the abomasum that are dissimilar from those of the rumen. Eleven lactating ewes were fed a TMR diet (forage:concentrate 40:60) supplemented with lipids for 54 days. Then, animals were slaughtered and rumen and abomasal digesta were sampled for DNA extraction and T-RFLP analysis. The number of T-RF was similar (P>0.10) in both compartments but, in most sheep, the abomasum showed a few unique fragments with a notable relative frequency (1.2-11.1%) that are compatible with species of the genus Spirochaeta (phylum Spirochaetes; 66bp with MspI), the family Holophagaceae (phylum Acidobacteria; 359bp with HhaI and 199bp with HaeIII) and the order Actinomycetales (phylum Actinobacteria; 133bp with MspI). Although most bacterial DNA in the abomasum comes from rumen microorganisms, the abundance of these unique fragments suggests that the corresponding microbes might potentially inhabit this compartment, which is consistent with the observed abomasal pH (≈3.7) and the ability of certain gut bacteria to tolerate this acidity. Our results would therefore support the hypothesis that particular bacterial populations may considerably colonize the abomasum. Further research however would be necessary to confirm their identification and determine their significance to ruminant nutrition and health.
Descripción1 página.-- Gut microbiology: from sequence to function (Aberdeen, Reino Unido, 16-19 junio, 2014).
URIhttp://hdl.handle.net/10261/99217
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