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Use of stomach tubing as an alternative to rumen cannulation to study the bacterial community in sheep and goats

AutorRamos Morales, Eva; Arco Pérez, Ana; Martín García, A. Ignacio; Yáñez Ruiz, David R.; Frutos, Pilar ; Hervás, Gonzalo
Fecha de publicaciónjun-2014
EditorRowett Institute of Nutrition and Health
CitaciónGut microbiology: from sequence to function 154 (P204) (2014)
ResumenRumeh cannulation is considered the reference method for collection of representative samples of rumen digesta and is widely used in ruminant nutrition research. However, it is not feasible in lactating ewes or goats, because of adverse effects on animal performance, which obliges to depend on less invasive techniques, such as stomach tubing. The aim of this work was to study whether collecting samples through cannula or stomach tube detect the differences in the rumen bacterial community between species (sheep and goats) and diets (forage and forage plus concentrate, 1:1). Four sheep and four goats, fitted with ruminal cannula, were fed either forage or forage plus concentrate, in two 15-d periods. At the end of each period, samples of rumen digesta were taken after feeding, first by stomach tube and immediately afterwards via rumen cannula. PCRDGGE analysis of the bacterial community was carried out. The similarity dendrograrn showed that, within period, animal species was the factor driving the grouping pattem, which was more evident in samples collected through cannula. Furthermore, diversity indexes were higher for goats than for sheep only when sampling via cannula. None of the techniques detected significant variations in the bacterial diversity when comparing the diets. Our results show that stomach tubing allows the collection of a highly diverse bacterial community that is able to detect some of the effects observed when sampling through cannula. However, further studies in other microbial groups, using high-throughput sequencing tools, are needed to explore difierences in the abundances of some microbial taxa.
Descripción1 página.-- Gut microbiology: from sequence to function (Aberdeen, Reino Unido, 16-19 junio, 2014).
Aparece en las colecciones: (IGM) Comunicaciones congresos
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