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Differential selectivity of protein modification by the cyclopentenone prostaglandins PGA1 and 15-deoxy-Delta12,14-PGJ2: role of glutathione.

AutorGayarre, Javier; Stamatakis, Konstantinos ; Renedo, Marta; Pérez-Sala, Dolores
Palabras claveProstaglandin
Posttranslational modification
Michael addition
Fecha de publicación1-jul-2014
ResumenCyclopentenone prostaglandins (cyPG) with antiinflammatory and antiproliferative properties have been envisaged as leads for the development of therapeutic agents. Because cyPG effects are mediated in part by the formation of covalent adducts with critical signaling proteins, it is important to assess the specificity of this interaction. By using biotinylated derivatives of 15-deoxy-D12,14-PGJ2 (15d-PGJ2-B) and PGA1(PGA1-B) we herein provide novel evidence for the differential selectivity of protein modification by distinct cyPG. The marked quantitative and qualitative differences in the binding of 15d-PGJ2-B and PGA1-B to cellular proteins were related to a differential reactivity in the presence of glutathione (GSH), both in vitro and in intact cells. Therefore GSH levels may influence not only the intensity but also the specificity of cyPG action.
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