English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/99133
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

All-integrated and highly sensitive paper based device with sample treatment platform for Cd2+ immunodetection in drinking/tap waters

AuthorsPons, Josefina; Merkoçi, Arben
Issue Date2013
PublisherAmerican Chemical Society
CitationAnalytical Chemistry 85(7): 3532-3538 (2013)
AbstractNowadays, the development of systems, devices, or methods that integrate several process steps into one multifunctional step for clinical, environmental, or industrial purposes constitutes a challenge for many ongoing research projects. Here, we present a new integrated paper based cadmium (Cd 2+) immunosensing system in lateral flow format, which integrates the sample treatment process with the analyte detection process. The principle of Cd2+ detection is based on competitive reaction between the cadmium-ethylenediaminetetraacetic acid-bovine serum albumin-gold nanoparticles (Cd-EDTA-BSA-AuNP) conjugate deposited on the conjugation pad strip and the Cd-EDTA complex formed in the analysis sample for the same binding sites of the 2A81G5 monoclonal antibody (mAb), specific to Cd-EDTA but not Cd2+ free, which is immobilized onto the test line. This platform operates without any sample pretreatment step for Cd2+ detection thanks to an extra conjugation pad that ensures Cd2+ complexation with EDTA and interference masking through ovalbumin (OVA). The detection and quantification limits found for the device were 0.1 and 0.4 ppb, respectively, these being the lowest limits reported up to now for metal sensors based on paper. The accuracy of the device was evaluated by addition of known quantities of Cd2+ to different drinking water samples and subsequent Cd2+ content analysis. Sample recoveries ranged from 95 to 105% and the coefficient of variation for the intermediate precision assay was less than 10%. In addition, the results obtained here were compared with those obtained with the well-established inductively coupled plasma emission spectroscopy (ICPES) and the analysis of certificate standard samples. © 2013 American Chemical Society.
URIhttp://hdl.handle.net/10261/99133
DOI10.1021/ac3034536
Identifiersdoi: 10.1021/ac3034536
issn: 0003-2700
e-issn: 1520-6882
Appears in Collections:(CIN2) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.