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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Barrera, Gloria | - |
dc.contributor.author | Williams, Trevor G. | - |
dc.contributor.author | Villamizar, Laura R. | - |
dc.contributor.author | Caballero, Primitivo | - |
dc.contributor.author | Simón, Oihane | - |
dc.date.accessioned | 2014-06-23T09:11:08Z | - |
dc.date.available | 2014-06-23T09:11:08Z | - |
dc.date.issued | 2013-06 | - |
dc.identifier.citation | IOBC-WPRS Bulletin 90: 133-136 (2013) | - |
dc.identifier.isbn | 978-92-9067-268-5 | - |
dc.identifier.issn | 1027-3115 | - |
dc.identifier.uri | http://hdl.handle.net/10261/98750 | - |
dc.description | Trabajo presentado en el IOBC-WPRS Working Group Insect Pathogens and Insect Parasitic Nematodes, celebrado en Zagreb del 16 al 20 de junio de 2013. | - |
dc.description.abstract | The Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different variants in SfCOL-wt a plaque assay was performed and ten distinct genotypes were identified (called A through J). SfCOL-A was the most prevalent (71±9%) and showed a PstI restriction profile identical to that of SfCOL-wt. The remaining nine genotypes presented genomic deletions of 3.8-21.8 Kb, located between nucleotides 11.436 and 33.883 in the Nicaraguan genotype of SfMNPV (SfMNPV-B). These deletions affected the region between open reading frames (ORFs) sf20 and sf33. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of mixtures of OBs and co-occluded mixed genotype OBs were consistently reduced in twogenotype mixtures involving equal proportions of SfCOL-A and one of three deletion genotypes (SfCOL-C, -D or –F). Speed of kill and OB production were improved only when the certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any of the component genotypes or mixtures thereof. We conclude that the SfCOL-wt population is structured to maximize the production of OBs in each infected host. | - |
dc.description.sponsorship | This study received financial support from COLCIENCIAS project 1753- 2011 and the projects AGL2008-05456-CO3-01 and AGL2011-30352-CO2-01 funded by the Spanish Ministry of Science and Technology. | - |
dc.publisher | International Organisation for Biological and Integrated Control of Noxious Animals and Plants | - |
dc.rights | openAccess | - |
dc.subject | Wild-type | - |
dc.subject | Genotypes | - |
dc.subject | Mixtures of OBs | - |
dc.subject | Colombia | - |
dc.subject | SfMNPV | - |
dc.subject | Phenotype | - |
dc.title | Deletion genotypes influence occlusion body potency and production infected by a Spodoptera frugiperda nucleopolyhedrovirus isolates from Colombia | - |
dc.type | comunicación de congreso | - |
dc.date.updated | 2014-06-23T09:11:08Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.type.coar | http://purl.org/coar/resource_type/c_5794 | es_ES |
item.openairetype | comunicación de congreso | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
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deletion_genotypes_Barrera.pdf | 1,77 MB | Adobe PDF | Visualizar/Abrir |
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