English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/98750
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título

Deletion genotypes influence occlusion body potency and production infected by a Spodoptera frugiperda nucleopolyhedrovirus isolates from Colombia

Autor Barrera, Gloria; Williams, Trevor G.; Villamizar, Laura R.; Caballero, Primitivo ; Simón, Oihane
Palabras clave Wild-type
Genotypes
Mixtures of OBs
Colombia
SfMNPV
Phenotype
Fecha de publicación jun-2013
EditorInternational Organisation for Biological and Integrated Control of Noxious Animals and Plants
Citación IOBC-WPRS Bulletin 90: 133-136 (2013)
ResumenThe Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different variants in SfCOL-wt a plaque assay was performed and ten distinct genotypes were identified (called A through J). SfCOL-A was the most prevalent (71±9%) and showed a PstI restriction profile identical to that of SfCOL-wt. The remaining nine genotypes presented genomic deletions of 3.8-21.8 Kb, located between nucleotides 11.436 and 33.883 in the Nicaraguan genotype of SfMNPV (SfMNPV-B). These deletions affected the region between open reading frames (ORFs) sf20 and sf33. The potency of SfCOL-A occlusion bodies (OBs) was 4.4-fold higher than SfCOL-wt OBs, whereas the speed of kill of SfCOL-A was similar to that of SfCOL-wt. Deletion genotype OBs were similarly or less potent than SfCOL-wt but six deletion genotypes were faster killing than SfCOL-wt. The potency of mixtures of OBs and co-occluded mixed genotype OBs were consistently reduced in twogenotype mixtures involving equal proportions of SfCOL-A and one of three deletion genotypes (SfCOL-C, -D or –F). Speed of kill and OB production were improved only when the certain genotype mixtures were co-occluded, although OB production was higher in the SfCOL-wt isolate than in any of the component genotypes or mixtures thereof. We conclude that the SfCOL-wt population is structured to maximize the production of OBs in each infected host.
Descripción Trabajo presentado en el IOBC-WPRS Working Group Insect Pathogens and Insect Parasitic Nematodes, celebrado en Zagreb del 16 al 20 de junio de 2013.
URI http://hdl.handle.net/10261/98750
ISBN 978-92-9067-268-5
ISSN1027-3115
Aparece en las colecciones: (IDAB) Libros y partes de libros
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
deletion_genotypes_Barrera.pdf1,77 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.