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Coordinated c-di-GMP repression of Salmonella motility through YcgR and cellulose

AuthorsZorraquino, Violeta ; García, Begoña ; Latasa Osta, Cristina ; Echeverz, Maite; Toledo-Arana, Alejandro ; Valle Turrillas, Jaione ; Lasa, Íñigo ; Solano Goñi, Cristina
Issue DateMar-2013
CitationESF-EMBO Conference on Bacterial Networks (2013)
AbstractCyclic di-GMP (c-di-GMP) is a secondary messenger that controls a variety of cellular processes including the switch between a biofilm and a planktonic bacterial lifestyle. This nucleotide binds to cellular effectors in order to exert its regulatory functions. In Salmonella, two proteins, BcsA and YcgR, both of them containing a c-di-GMP binding PilZ domain, are the only known c-di-GMP receptors. BcsA, upon c-di-GMP binding, synthesizes cellulose, the main exopolysaccharide of the biofilm matrix. YcgR is dedicated to c-di-GMP dependent inhibition of motility through its interaction with flagellar motor proteins. However, previous evidences indicate that in the absence of YcgR there is still an additional element that mediates motility impairment under high c-di-GMP levels. Here, we uncover that cellulose per se is the factor that further promotes inhibition of bacterial motility once high c-di-GMP contents drive the activation of a sessile lifestyle. Inactivation of different genes of the bcsABZC operon, mutation of the conserved residues in the RxxxR motif of the BcsA PilZ domain or degradation of the cellulose produced by BcsA rescued the motility defect of ∆ycgR strains in which high c-di-GMP levels were reached through the overexpression of diguanylate cyclases. High c-di-GMP levels provoked cellulose accumulation around cells that impeded flagella rotation probably by means of steric hindrance, without affecting flagella gene expression, exportation or assembly. Our results highlight the relevance of cellulose in Salmonella lifestyle switching as an architectural element that is both essential for biofilm development and required, in collaboration with YcgR, for complete motility inhibition.
DescriptionTrabajo presentado en la ESF-EMBO Conference on Bacterial Networks, celebrada en Pultusk (Polonia) del 16 al 21 de marzo de 2013.
Appears in Collections:(IDAB) Comunicaciones congresos
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