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Título

Assesment of treatments against Staphylococcus aureus biofilms using in vivo 18F-FDG-PET in an animal model

Autor Collantes, María; Garrido, Victoria ; Barberán, Montserrat; Arbizu, Javier; Amorena Zabalza, Beatriz ; Abadía, S.; Ecay, M.; Grilló, María Jesús ; Peñuelas, Iván
Fecha de publicación 2013
EditorEuropean Association of Nuclear Medicine
Citación 26th Annual Congress of thr European Association of Nuclear Medicine (2013)
ResumenBiofilms are aggregates of microorganisms that may be formed on different surfaces as medical implants. Their appearance is associated to almost undetectable and persistent infections that hardly respond to antibiotic treatments. Consequently, the development of new antibiotic therapies and of adequate techniques to confirm their effectiveness is of utmost importance.
[Aim] to evaluate the feasibility of 18F-FDG-PET imaging for in vivo monitoring of bacterial biofilm infections and quantifying the response to antibiotic treatments in an animal model.
[Methodology] sealed catheters of VialonTM biomaterial were pre-colonized with Staphylococcus aureus ATCC15981 and subcutaneously implanted in CD1 mice (n=14). Half of these animals (n=7) received oral rifampicin (0.5 mg/day) for 7 days. The infection was monitored in vivo by 18F-FDG-PET imaging at days 1, 7 and 14 after catheter implantation. For this, an 18F-FDG-PET (18,3±1,6 MBq) was injected in the tail vein, and after 60 minutes under continuous isoflurane anesthesia, a static PET image was performed. 18F-FDG uptake was quantified by means of SUV, drawing volumes of interest over the catheters and lymph nodes. In parallel, an additional group of untreated mice (n=9) was used to determine both the number of colony forming units (CFU) per catheter and the histopathological changes (i.e. presence of inflammatory cells and bacteria) in the surrounding area of the catheter.
[Results] 18F-FDG-PET produced a quantifiable signal already at 1 day post-implant. The signal was found associated with the appearance of bacteria and polymorphonuclear cells in the area of the catheter. Moreover, a high 18F-FDG-PET uptake in lymph nodes was observed in the non-treated group at days 7 and 14. At day 7, the rifampicin-treated group showed a statistically significant reduction in both SUV values (p=0.006) and number of viable CFU (p<0.001) in catheters, and lymph node images became negative. At day 14 (one week after treatment was stopped), the SUV values of catheter and lymph nodes increased in the treated group, reaching levels similar to those of the control group, compatible with the growth of surviving bacteria.
[Conclusions] 18F-FDG-PET could be an innovative and useful technique to detect in vivo biofilm infections, in follow-up studies and evaluation of the efficacy of antibiotic therapies in living mice.
Descripción Trabajo presentado en la 26th European Association of Nuclear Medicine (EANM) annual congress, celebrada en Lyon (Francia) en 2013.
URI http://hdl.handle.net/10261/98737
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