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2D-NMR demonstration of lignin removal from wood and non-wood plant feedstocks by fungal versatile peroxidase

AuthorsFernández-Fueyo, Elena ; Rencoret, Jorge ; Berbís, Manuel Álvaro ; Ruiz-Dueñas, F. J. ; Gutiérrez Suárez, Ana ; Río Andrade, José Carlos del ; Jiménez-Barbero, Jesús ; Hammel, Kenneth E.; Martínez, Ángel T.
Versatile peroxidase
Veratryl alcohol
Issue Date24-Jun-2014
PublisherCSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS)
CitationProceedings of the 13th European Workshop on Lignocellulosics and Pulp, pp. 347-350 (2014)
AbstractThe ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escherichia coli and "in vitro" folded. Two-dimensional nuclear magnetic resonance (NMR) of the treated materials, swollen in dimethylsulfoxide-d6, revealed a reduction in the number of lignin side-chain linkages after both treatments. However, a significant removal of lignin by the enzymatic treatment was only observed in the case of eucalypt wood, as shown by the decrease of lignin aromatic signals referred to carbohydrate anomeric signals.
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