English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/98699
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Use of new EST markers to elucidate the genetic differences in grain protein content between European and North American two-rowed malting barleys

AuthorsMoralejo, María Ángeles; Swanston, J. S.; Muñoz, Pilar; Prada, Darío; Elía, Mónica; Russell, Joanne R.; Ramsay, Luke D.; Cistué Sola, Luis ; Codesal, Primitiva; Casas Cendoya, Ana María ; Romagosa, Ignacio; Powell, Wayne; Molina-Cano, José Luis
KeywordsCrops
Enzyme kinetics
Proteins
Genetic engineering
Growth kinetics
Genes
Issue DateDec-2004
PublisherSpringer
CitationTAG, Theoretical and applied genetics, Theoretische und angewandte Genetik 110 (1): 116-125 (2004)
AbstractA population comprising 102 doubled haploid lines were produced from a cross between Beka, a barley cultivar widely grown in Spain, and Logan, a north American cultivar with inherently low protein content, a character considered to derive from the cultivar Karl. The intentions were to determine whether low-nitrogen malting barleys could be developed in Spain, and if genetic factors that influenced protein content were similarly expressed in widely diverse environments, i.e. northeastern Spain and eastern Scotland. An extensive map comprising 187 molecular markers was developed. Expressed sequence-tagged- derived markers were used in addition to anonymous simple sequence repeats to determine the potential for identifying candidate genes for quantitative trait loci (QTLs), and 22 such markers were mapped for the first time. There was transgressive segregation for both yield and protein content, and the gene for low protein from Logan was not expressed in the Scottish environment. In 2002, high yield was associated with earlier heading date in Spain, while late heading at the Scottish site was associated with greater lodging and lower thousand-kernel weight. These appeared to be possible pleiotropic effects of a factor detected on chromosome 2H. Using information from a consensus map, it was shown that this locus on 2H was in the region of the photoperiod response gene Eam6. A QTL explaining 18% of the variation in grain protein content was detected on chromosome 5H in a region in which a gene for nitrate reductase was previously observed. No effect on grain protein was associated with chromosome 6H, which has been suggested as the location of the low protein gene from Karl. However, it is likely that Karl contained more than one genetic factor reducing protein, and we postulate that the gene on 6H may have been lost during the breeding of Logan.
Publisher version (URL)http://dx.doi.org/10.1007/s00122-004-1805-7
URIhttp://hdl.handle.net/10261/98699
DOI10.1007/s00122-004-1805-7
Identifiersdoi: 10.1007/s00122-004-1805-7
issn: 0040-5752
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.