English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/98572
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Cloning, functional expression and characterization of Mesorhizobium loti arylamine N-acetyltransferases: rhizobial symbiosis supplies leguminous plants with the xenobiotic N-acetylation pathway

AutorRodrigues-Lima, Fernando; Dairou, Julien; Diaz, Clara L.; Rubio Luna, María Carmen ; Sim, Edith; Spaink, Herman P.; Dupret, Jean-Marie
Palabras claveFabaceae
Eukaryota
Bacteria (microorganisms)
Xenobiotic agent
Recombinant enzyme
Pesticide
Isoenzyme
Arylamine acetyltransferase
Aniline derivative
Lotus corniculatus var. japonicus
Mesorhizobium loti
Rhizobium
Mesorhizobium
Fecha de publicaciónabr-2006
EditorBlackwell Publishing
CitaciónMolecular Microbiology 60 (2): 505-512 (2006)
ResumenArylamine N-acetyltransferases (NATs) are xenobiotic-metabolizing enzymes involved in the detoxification of numerous aromatic chemicals. The NATdependent N-acetylation pathway has not previously been detected in plants. We demonstrate here the occurrence of the NAT-dependent pathway in leguminous plants, due to symbiosis with Mesorhizobium loti. We cloned two NAT enzymes from M. loti and showed that these two recombinant enzymes catalysed the N-acetylation of several known NAT substrates, including aniline-derived pesticide residues. We also demonstrate the existence of a functional NAT-dependent acetylation pathway in the root nodules of Lotus japonicus inoculated with M. loti. M. loti is the first non-eukaryotic organism shown to express two catalytically active NAT isoforms. This work also provides the first evidence for acquisition of a xenobiotic detoxification pathway by a plant through symbiosis with a soil microbe.
Versión del editorhttp://dx.doi.org/10.1111/j.1365-2958.2006.05114.x
URIhttp://hdl.handle.net/10261/98572
DOI10.1111/j.1365-2958.2006.05114.x
Identificadoresdoi: 10.1111/j.1365-2958.2006.05114.x
issn: 0950-382X
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.