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Determination of capsaicin and dihydrocapsaicin in Capsicum fruits by liquid chromatography-electrospray/time-of-flight mass spectrometry

AutorGarcés-Claver, Ana ; Arnedo Andrés, María Soledad; Abadía Bayona, Javier ; Gil Ortega, Ramiro; Álvarez-Fernández, Ana
Palabras claveMass spectrometry
Liquid chromatography
Fecha de publicación13-dic-2006
EditorAmerican Chemical Society
CitaciónJournal of Agricultural and Food Chemistry 54 (25): 9303-9311 (2006)
ResumenA simple, highly selective, sensitive, and reproducible liquid chromatography-electrospray ionization/ time-of-flight mass spectrometry method has been developed for the direct and simultaneous determination of capsaicin and dihydrocapsaicin in Capsicum fruit extracts. Capsaicin and dihydrocapsaicin are the two major members of the so-called capsaicinoid family, which includes other minor analogues, and usually account for at least 90% of the pungency trait in Capsicum fruits. Chromatographic separation of capsaicin and dihydrocapsaicin was achieved with a reversed-phase chromatography column, using a gradient of methanol and water. Quantification was done using as an internal standard (4,5-dimethoxybenzyl)-4-methyloctamide, a synthetic capsaicin analogue not found in nature. Analytes were base-peak resolved in less than 16 min, and limits of detection were 20 pmol for capsaicin and 4 pmol for dihydrocapsaicin. The intraday repeatability values were lower than 0.5 and 12% for retention time and peak area, respectively, whereas the interday repeatability values were lower than 0.6 and 14% for retention time and peak area, respectively. Analyte recoveries found were 86 and 93% for capsaicin and dihydrocapsaicin, respectively. The method developed has been applied to the identification and quantification of capsaicin and dihydrocapsaicin in fruit extracts from different Capsicum genotypes, and concentrations found ranged from 2 to 6639 mg kg-1. © 2006 American Chemical Society.
Versión del editorhttp://dx.doi.org/10.1021/jf0620261
Identificadoresdoi: 10.1021/jf0620261
issn: 0021-8561
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