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Nickel-resistance determinants in acidiphilium sp. Pm identified by genome-wide functional screening

AuthorsSan Martín-Uriz, Patxi; Mirete, Salvador ; Alcolea, Pedro J. ; Gómez, Manuel José ; Amils, Ricardo ; González-Pastor, José Eduardo
Issue Date16-Apr-2014
PublisherPublic Library of Science
CitationPLoS ONE 9 (2014)
AbstractAcidiphilium spp. are conspicuous dwellers of acidic, metal-rich environments. Indeed, they are among the most metalresistant organisms; yet little is known about the mechanisms behind the metal tolerance in this genus. Acidiphilium sp. PM is an environmental isolate from Rio Tinto, an acidic, metal-laden river located in southwestern Spain. The characterization of its metal resistance revealed a remarkable ability to tolerate high Ni concentrations. Here we report the screening of a genomic library of Acidiphilium sp. PM to identify genes involved in Ni resistance. This approach revealed seven different genes conferring Ni resistance to E. coli, two of which form an operon encoding the ATP-dependent protease HslVU (ClpQY). This protease was found to enhance resistance to both Ni and Co in E. coli, a function not previously reported. Other Niresistance determinants include genes involved in lipopolysaccharide biosynthesis and the synthesis of branched amino acids. The diversity of molecular functions of the genes recovered in the screening suggests that Ni resistance in Acidiphilium sp. PM probably relies on different molecular mechanisms. © 2014 San Martin-Uriz et al.
Description11 p.-1 tab.-5 fig.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0095041
Appears in Collections:(CIB) Artículos
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