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Título

Muscle-specific overexpression of the catalytic subunit of dna polymerase γ induces pupal lethality in Drosophila melanogaster

Autor Martínez-Azorín, Francisco; Calleja, Manuel; Hernández-Sierra, Rosana ; Farr, Carol L.; Kaguni, Laurie S.; Garesse, Rafael
Palabras clave Mitochondria
Oxidative phosphorylation
Drosophila melanogaster
DNA polymerase
Apoptosis
Fecha de publicación 2013
EditorJohn Wiley & Sons
Citación Archives of Insect Biochemistry and Physiology 83(3): 127-137 (2013)
ResumenWe show the physiological effects and molecular characterization of overexpression of the catalytic core of mitochondrial DNA (mtDNA) polymerase (pol γ-α) in muscle of Drosophila melanogaster. Muscle-specific overexpression of pol γ-α using the UAS/GAL4 (where UAS is upstream activation sequence) system produced more than 90% of lethality at the end of pupal stage at 25°C, and the survivor adult flies showed a significant reduction in life span. The survivor flies displayed a decreased mtDNA level that is accompanied by a corresponding decrease in the levels of the nucleoid-binding protein mitochondrial transcription factor A (mtTFA). Furthermore, an increase in apoptosis is detected in larvae and adults overexpressing pol γ-α. We suggest that the pupal lethality and reduced life span of survivor adult flies are both caused mainly by massive apoptosis of muscle cells induced by mtDNA depletion. © 2013 Wiley Periodicals, Inc.
URI http://hdl.handle.net/10261/98063
DOI10.1002/arch.21101
Identificadoresdoi: 10.1002/arch.21101
issn: 0739-4462
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