English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/97926
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


HvFT1 polymorphism and effect—survey of barley germplasm and expression analysis

AuthorsLoscos Aranda, Jorge ; Igartua Arregui, Ernesto ; Contreras-Moreira, Bruno ; Gracia Gimeno, María Pilar ; Casas Cendoya, Ana María
Flowering time
Copy number variation
Issue Date6-Jun-2014
PublisherFrontiers Media
CitationLoscos J, Igartua E, Contreras-Moreira B, Gracia MP, Casas AM. HvFT1 polymorphism and effect—survey of barley germplasm and expression analysis. Frontiers in Plant Science 5: 251 (2014)
AbstractFlowering time in plants is a tightly regulated process. In barley (Hordeum vulgare L.), HvFT1, ortholog of FLOWERING LOCUS T, is the main integrator of the photoperiod and vernalization signals leading to the transition from vegetative to reproductive state of the plant. This gene presents sequence polymorphisms affecting flowering time in the first intron and in the promoter. Recently, copy number variation (CNV) has been described for this gene. An allele with more than one copy was linked to higher gene expression, earlier flowering, and an overriding effect of the vernalization mechanism. This study aims at (1) surveying the distribution of HvFT1 polymorphisms across barley germplasm and (2) assessing gene expression and phenotypic effects of HvFT1 alleles. We analyzed HvFT1 CNV in 109 winter, spring, and facultative barley lines. There was more than one copy of the gene (2–5) only in spring or facultative barleys without a functional vernalization VrnH2 allele. CNV was investigated in several regions inside and around HvFT1. Two models of the gene were found: one with the same number of promoters and transcribed regions, and another with one promoter and variable number of transcribed regions. This last model was found in Nordic barleys only. Analysis of HvFT1 expression showed that association between known polymorphisms at the HvFT1 locus and the expression of the gene was highly dependent on the genetic background. Under long day conditions the earliest flowering lines carried a sensitive PpdH1 allele. Among spring cultivars with different number of copies, no clear relation was found between CNV, gene expression and flowering time. This was confirmed in a set of doubled haploid lines of a population segregating for HvFT1 CNV. Earlier flowering in the presence of several copies of HvFT1 was only seen in cultivar Tammi, which carries one promoter, suggesting a relation of gene structure with its regulation. HvCEN also affected to a large extent flowering time.
Description15 p., 6 fig., 4 tab. Copyright © 2014 Loscos, Igartua, Contreras-Moreira, Gracia and Casas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Publisher version (URL)http://dx.doi.org/10.3389/fpls.2014.00251
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
CasasAM_FrontPlantSci_2014_525_1.pdf3,75 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.