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dc.contributor.authorAlmagro, Goizeder-
dc.contributor.authorBaroja-Fernández, Edurne-
dc.contributor.authorMuñoz Pérez, Francisco José-
dc.contributor.authorBahaji, Abdellatif-
dc.contributor.authorEtxebarría, E.-
dc.contributor.authorLi, Jun-
dc.contributor.authorMontero, Manuel-
dc.contributor.authorHidalgo, Maite-
dc.contributor.authorSesma, María Teresa-
dc.contributor.authorPozueta Romero, Javier-
dc.identifierdoi: 10.4161/psb.20601-
dc.identifierissn: 1559-2316-
dc.identifier.citationPlant Signaling and Behavior 7(7): 799-802 (2012)-
dc.description.abstractSucrose synthase (SuSy) catalyzes the reversible conversion of sucrose and NDP into the corresponding nucleotidesugars and fructose. The Arabidopsis genome possesses six SUS genes (AtSUS1-6) that code for proteins with SuSy activity. As a first step to investigate optimum fructose and UDP-glucose (UDPG) concentrations necessary to measure maximum sucrose-producing SuSy activity in crude extracts of Arabidopsis, in this work we performed kinetic analyses of recombinant AtSUS1 in two steps: (1) SuSy reaction at pH 7.5, and (2) chromatographic measurement of sucrose produced in step 1. These analyses revealed a typical Michaelis-Menten behavior with respect to both UDPG and fructose, with Km values of 50 μM and 25 μM, respectively. Unlike earlier studies showing the occurrence of substrate inhibition of UDP-producing AtSUS1 by fructose and UDP-glucose, these analyses also revealed no substrate inhibition of AtSUS1 at any UDPG and fructose concentration. By including 200 μM fructose and 1 μM UDPG in the SuSy reaction assay mixture, we found that sucrose-producing SuSy activity in leaves and stems of Arabidopsis were exceedingly higher than previously reported activities. Furthermore, we found that SuSy activities in organs of the sus1/sus2/sus3/sus4 mutant were ca. 80-90% of those found in WT plants. © 2012 Landes Bioscience.-
dc.description.sponsorshipThis research was partially supported by the grant BIO2010-18239 from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) and by Iden Biotechnology S.L. G.A. acknowledges a fellowship from the Public University of Navarra.-
dc.publisherLandes Bioscience-
dc.titleNo evidence for the occurrence of substrate inhibition of Arabidopsis thaliana sucrose synthase-1 (AtSUS1) by fructose and UDP-glucose-
dc.description.versionPeer Reviewed-
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