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dc.contributor.author | Almagro, Goizeder | - |
dc.contributor.author | Baroja-Fernández, Edurne | - |
dc.contributor.author | Muñoz Pérez, Francisco José | - |
dc.contributor.author | Bahaji, Abdellatif | - |
dc.contributor.author | Etxebarría, E. | - |
dc.contributor.author | Li, Jun | - |
dc.contributor.author | Montero, Manuel | - |
dc.contributor.author | Hidalgo, Maite | - |
dc.contributor.author | Sesma, María Teresa | - |
dc.contributor.author | Pozueta Romero, Javier | - |
dc.date.accessioned | 2014-06-05T11:26:09Z | - |
dc.date.available | 2014-06-05T11:26:09Z | - |
dc.date.issued | 2012 | - |
dc.identifier | doi: 10.4161/psb.20601 | - |
dc.identifier | issn: 1559-2316 | - |
dc.identifier.citation | Plant Signaling and Behavior 7(7): 799-802 (2012) | - |
dc.identifier.uri | http://hdl.handle.net/10261/97823 | - |
dc.description.abstract | Sucrose synthase (SuSy) catalyzes the reversible conversion of sucrose and NDP into the corresponding nucleotidesugars and fructose. The Arabidopsis genome possesses six SUS genes (AtSUS1-6) that code for proteins with SuSy activity. As a first step to investigate optimum fructose and UDP-glucose (UDPG) concentrations necessary to measure maximum sucrose-producing SuSy activity in crude extracts of Arabidopsis, in this work we performed kinetic analyses of recombinant AtSUS1 in two steps: (1) SuSy reaction at pH 7.5, and (2) chromatographic measurement of sucrose produced in step 1. These analyses revealed a typical Michaelis-Menten behavior with respect to both UDPG and fructose, with Km values of 50 μM and 25 μM, respectively. Unlike earlier studies showing the occurrence of substrate inhibition of UDP-producing AtSUS1 by fructose and UDP-glucose, these analyses also revealed no substrate inhibition of AtSUS1 at any UDPG and fructose concentration. By including 200 μM fructose and 1 μM UDPG in the SuSy reaction assay mixture, we found that sucrose-producing SuSy activity in leaves and stems of Arabidopsis were exceedingly higher than previously reported activities. Furthermore, we found that SuSy activities in organs of the sus1/sus2/sus3/sus4 mutant were ca. 80-90% of those found in WT plants. © 2012 Landes Bioscience. | - |
dc.description.sponsorship | This research was partially supported by the grant BIO2010-18239 from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) and by Iden Biotechnology S.L. G.A. acknowledges a fellowship from the Public University of Navarra. | - |
dc.publisher | Landes Bioscience | - |
dc.rights | closedAccess | - |
dc.title | No evidence for the occurrence of substrate inhibition of Arabidopsis thaliana sucrose synthase-1 (AtSUS1) by fructose and UDP-glucose | - |
dc.type | artículo | - |
dc.identifier.doi | 10.4161/psb.20601 | - |
dc.date.updated | 2014-06-05T11:26:09Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.identifier.pmid | 22751299 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | No Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | artículo | - |
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