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Title

Dissection of Host Cell Signal Transduction during Acinetobacter baumannii - Triggered Inflammatory Response

AuthorsMarch, Catalina; Regueiro, Verónica; Llobet Brossa, Enrique; Moranta, David; Morey, Pau; Garmendia, Juncal ; Bengoechea, José Antonio
Issue Date7-Apr-2010
PublisherPublic Library of Science
CitationPLoS ONE 5(4): e10033 (2010)
AbstractInfected airway epithelial cells up-regulate the expression of chemokines, chiefly IL-8, and antimicrobial molecules including β-defensins (BD). Acinetobacter baumannii is a cause of hospital-acquired pneumonia. We examined whether A. baumannii induced the expressions of IL-8 and BD2 by airway epithelial cells and the receptors implicated in bacterial detection. A549 and human primary airway cells released IL-8 upon infection. A. baumannii-infected cells also increased the expression of BD2 which killed A. baummannii strains. IL-8 induction was via NF-κB and mitogen-activated kinases p38 and p44/42-dependent pathways. A. baumannii engaged Toll-like receptor (TLR) 2 and TLR4 pathways and A549 cells could use soluble CD14 as TLRs co-receptor. A. baumannii lipopolysaccharide stimulated IL-8 release by A549 cells and sCD14 facilitated the recognition of the lipopolysaccharide. Mass spectrometry analysis revealed that A. baumannii lipid A structure matches those with endotoxic potential. These results demonstrate that airway epithelial cells produce mediators important for A. baumannii clearance.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0010033
URIhttp://hdl.handle.net/10261/97805
DOI10.1371/journal.pone.0010033
Identifiersdoi: 10.1371/journal.pone.0010033
issn: 1932-6203
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