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Identification of culturable bacteria present in haemodialysis water and fluid

AuthorsGomila, Margarita; Gascó, Joan M.; Busquets, Antonio; Gil, José; Bernabeu, Rosario; Buades, Juan Manuel; Lalucat, Jorge
Issue Date2005
CitationFEMS Micriobiology Ecology 52(1): 101-114 (2005)
AbstractWater used to prepare haemodialysis fluid is not sterile, and its microbiological control is important for the prevention of haemodialysis- associated illness. Bacterial populations inhabiting a distribution system for haemodialysis water were studied over an 18-month period. 203 planktonic bacteria isolated on R2A medium were identified by restriction analysis and sequencing of 16S rRNA gene. A diverse bacterial community was detected, containing predominantly Gram-negative members of the Alphaproteobacteria and Betaproteobacteria, as well as representatives of the genus Mycobacterium. Ecological and clinical consequences are discussed: bacteria from the genera Novosphingobium, Pseudomonas and Sphingomonas have been described in the build-up of biofilms, and others like Acinetobacter, Mycobacterium or Brevibacterium may represent a health risk to patients under haemodialysis treatment. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Identifiersdoi: 10.1016/j.femsec.2004.10.015
issn: 0168-6496
Appears in Collections:(IMEDEA) Artículos
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