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Enhanced Water and Cryoprotectant Permeability of Porcine Oocytes After Artificial Expression of Human and Zebrafish Aquaporin-3 Channels

AuthorsMorató, Roser; Chauvigné, François ; Novo, Segi; Bonet, Sergi; Cerdà, Joan
Issue DateMay-2014
CitationMolecular Reproduction and Development 81(5): 450-461 (2014)
AbstractOne of the major obstacles for the vitrification of mature porcine oocytes with ethylene glycol is their low permeability to this cryoprotectant, which results in osmotic stress-induced cell damage and low survival. Pig blastocysts, on the other hand, show enhanced water and cryoprotectant permeability, which has been related to the transcriptional activation of aquaporin-3 (AQP3) channels at this stage of development. In this study, we asked if expression of cRNAs encoding two aquaglyceroporins, human AQP3 (hAQP3) or the zebrafish Aqp3b-T85A mutant, in porcine oocytes can increase their permeability. Microinjection of germinal-vesicle-stage oocytes with enhanced green fluorescent protein (EGFP) or AQP3 cRNAs resulted in the expression of the corresponding proteins in ∼26% of the metaphase-II stage oocytes at 40-44hr of in vitro culture; co-injection of EGFP cRNA appeared to be a suitable marker for oocyte selection since all EGFP-positive oocytes also expressed the corresponding aquaporin. Using this method, we found that mature oocytes co-expressing EGFP and hAQP3 or EGFP and Aqp3b-T85A showed approximately a twofold increase of the hydraulic conductivity (Lp) with respect non-injected or EGFP alone-injected oocytes in a 0.43M sucrose or 1.3M ethylene glycol solution, whereas the ethylene glycol permeability (PEG) of EGFP+hAQP3 and EGFP+Aqp3b-T85A oocytes was 6.7- and 12-fold higher, respectively, than control oocytes. These data demonstrate that the artificial expression of aquaglyceroporins in porcine metaphase-II oocytes improves their permeability, and that the zebrafish Aqp3b-T85A mutant is more efficient than the human channel at increasing the oocyte permeability to ethylene glycol. © 2014 Wiley Periodicals, Inc.
Description12 pages, 5 figures, 4 tables
Publisher version (URL)https://doi.org/10.1002/mrd.22310
Identifiersdoi: 10.1002/mrd.22310
issn: 1040-452X
e-issn: 1098-2795
Appears in Collections:(ICM) Artículos
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