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Title

Induced inaccessibility in pea to powdery mildew: insights gained from the use of inhibitors

AuthorsMoral Moral, Juan ; Rubiales, Diego ; Prats, Elena
Issue DateMay-2013
PublisherInternational Legume Society
CitationFirst Legume Society Conference (2013)
AbstractThe term induced inaccessibility has been adopted to describe situations where early failed attacks by biotrophic pathogens to plant cells increase resistance to later attacks in that and neighboring cells. This kind of resistance widely relay in penetration resistance events however the particular cellular and molecular bases remain unknown. In the present study, we investigated the role of de novo protein formation and cytoskeleton assembly on induce d inaccessibility of pea (Pisum sativum) cells. For this aim, we sequentially inoculated pea leaves of the susceptible cv. Mesire, with an inappropriate powdery mildew species (Blumeria graminisf. sp. avenae), which causes a non2host interaction and then with pea powdery mildew (Erysiphe pisi). The epidermal pea cells in which the inappropriate powdery mildew failed to penetrate a nd their neighboring cells showed inaccessibility to subsequent infections by the pea powdery mildew. However, when the pea plants were treated with cytochalasin, an specific inhibitor of actin polymerization and cytoesqueleton formation, or cicloheximide, a protein synthesis inhibitor, the inaccessibility were greatly repressed. Microscopic and proteomics approaches are being carried out to understand how this events influence the inaccessibility in pea.
DescriptionPóster presentado en la First Legume Society Conference, celebrada en Novi Sad (Serbia) del 9 al 11 de mayo de 2013.
URIhttp://hdl.handle.net/10261/97582
Appears in Collections:(IAS) Comunicaciones congresos
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