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Título

The white-rot fungus Pleurotus ostreatus secretes laccase isozymes with different substrate specificities

AutorMansur, Mariana; Arias Fernández, Mª E.; Copa-Patiño, J.L.; Flärdh, María; González, Aldo E.
Palabras clavePhenoloxidase
Pleurotus
N-terminal sequence
Isozyme
Guaiacol
ABTS
Fecha de publicaciónjun-2003
EditorMycological Society of America
CitaciónMycologia 95 (6) 1013- 1020 (2003)
ResumenFour laccase isozymes (LCC1, LCC2, LCC3 and LCC4) synthesized by Pleurotus ostreatus strain V-184 were purified and characterized. LCC1 and LCC2 have molecular masses of about 60 and 65 kDa and exhibited the same pI value (3.0). Their N termini were sequenced, revealing the same amino acid sequence and homology with laccases from other microorganisms. Laccases LCC3 and LCC4 were characterized by SDS-PAGE, estimating their molecular masses around 80 and 82 kDa, respectively. By native isoelectrofocusing, their pI values were 4.7 and 4.5, respectively. When staining with ABTS and guaiacol in native polyacrilamide gels, different specificities were observed for LCC1/LCC2 and LCC3/ LCC4 isozymes.
Descripción8 p.-2 tab.-5 fig.
Versión del editorhttp://www.mycologia.org/content/95/6/1013.full
URIhttp://hdl.handle.net/10261/97404
ISSN0027-5514
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