Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/97334
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Distribution of genes involved in sialic acid utilization in strains of Haemophilus parasuis |
Autor: | Martínez, Verónica; Soler-Llorens, Pedro; Moleres, Javier CSIC ; Garmendia, Juncal CSIC ORCID ; Aragón, Virginia | Fecha de publicación: | 16-may-2012 | Editor: | Society for General Microbiology | Citación: | Microbiology 158(8): 2117-2124 (2012) | Resumen: | Haemophilus parasuis is a porcine respiratory pathogen, well known as the aetiological agent of Glässer's disease. H. parasuis comprises strains of different virulence, but the virulence factors of this bacterium are not well defined. A neuraminidase activity has been previously detected in H. parasuis, but the role of sialylation in the virulence of this bacterium has not been studied. To explore the relationship between sialic acid (Neu5Ac) and virulence, we assessed the distribution of genes involved in sialic acid metabolism in 21 H. parasuis strains from different clinical origins (including nasal and systemic isolates). The neuraminidase gene nanH, together with CMPNeu5Ac synthetase and sialyltransferase genes neuA, siaB and lsgB, were included in the study. Neuraminidase activity was found to be common in H. parasuis isolates, and the nanH gene from 12 isolates was expressed in Escherichia coli and further characterized. Sequence analysis showed that the NanH predicted protein contained the motifs characteristic of the catalytic site of sialidases. While an association between the presence of nanH and the different origins of the strains was not detected, the lsgB gene was predominantly present in the systemic isolates, and was not amplified from any of the nasal isolates tested. Analysis of the lipooligosaccharide (LOS) from reference strains Nagasaki (virulent, lsgB +) and SW114 (non-virulent, lsgB -) showed the presence of sialic acid in the LOS from the Nagasaki strain, supporting the role of sialylation in the virulence of this bacterial pathogen. Further studies are needed to clarify the role of sialic acid in the pathogenicity of H. parasuis. © 2012 SGM. | URI: | http://hdl.handle.net/10261/97334 | DOI: | 10.1099/mic.0.056994-0 | Identificadores: | doi: 10.1099/mic.0.056994-0 issn: 1350-0872 e-issn: 1465-2080 |
Aparece en las colecciones: | (IDAB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
31
checked on 20-abr-2024
WEB OF SCIENCETM
Citations
29
checked on 27-feb-2024
Page view(s)
349
checked on 23-abr-2024
Download(s)
92
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.