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Título

MM1, a temperate bacteriophage of the type 23F Spanish/USA multiresistant epidemic clone of Streptococcus pneumoniae: Structural analysis of the site-specific integration system

Autor Gindreau, Emmanuel; López, Rubens; García, Pedro ; García, Pedro
Fecha de publicación sep-2000
EditorAmerican Society for Microbiology
Citación Journal of Virology 74 (17) 7803- 7813 (2000)
ResumenWe have characterized a temperate phage (MM1) from a clinical isolate of the multiply antibiotic-resistant Spanish/American 23F Streptococcus pneumoniae clone (Spain(23F)-1 strain). The 40-kb double-stranded genome of MM1 has been isolated as a DNA-protein complex. The use of MM1 DNA as a probe revealed that the phage genome is integrated in the host chromosome. The host and phage attachment sites, attB and attP, respectively, have been determined. Nucleotide sequencing of the attachment sites identified a 15-bp core site (5'-TTATA ATTCATCCGC-3') that has not been found in any bacterial genome described so far. Sequence information revealed the presence of an integrase gene (int), which represents the first identification of an integrase in the pneumococcal system. A 1.5-kb DNA fragment embracing attP and the int gene contained all of the genetic information needed for stable integration of a nonreplicative plasmid into the attB site of a pneumococcal strain. This vector will facilitate the introduction of foreign genes into the pneumococcal chromosome. Interestingly, DNAs highly similar to that of MM1 have been detected in several clinical pneumococcal isolates of different capsular types, suggesting a widespread distribution of these phages in relevant pathogenic strains.
Descripción 11 p.-1 tab.-8 fig.
Versión del editorhttp://dx.doi.org/10.1128/JVI.74.17.7803-7813.2000
URI http://hdl.handle.net/10261/97297
DOI10.1128/JVI.74.17.7803-7813.2000
ISSN0022-538X
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