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dc.contributor.authorHerrera, Mercedes-
dc.contributor.authorAlba-Castellón, L.-
dc.contributor.authorSilva, Javier-
dc.contributor.authorGarcía, Vanesa-
dc.contributor.authorGarcía, José M.-
dc.contributor.authorGil, Beatriz-
dc.contributor.authorLarriba, María Jesús-
dc.contributor.authorCasal, J. Ignacio-
dc.contributor.authorGarcía de Herreros, Antonio-
dc.contributor.authorBonilla, Félix-
dc.contributor.authorPeña, Cristina-
dc.identifier.citationInternational Journal of Cancer 134(12): 2984-2990 (2014)es_ES
dc.descriptionet al.es_ES
dc.description.abstractSnail1 is a transcriptional factor that plays an important role in epithelial–mesenchymal transition and in the acquisition of invasive properties by epithelial cells. In colon tumors, Snail1 expression in the stroma correlates with lower specific survival of cancer patients. However, the role(s) of Snail1 expression in stroma and its association with patients' survival have not been determined. We used human primary carcinoma-associated fibroblasts (CAFs) or normal fibroblasts (NFs) and fibroblast cell lines to analyze the effects of Snail1 expression on the protumorigenic capabilities in colon cancer cells. Snail1 expression was higher in CAFs than in NFs and, as well as α-SMA, a classic marker of activated CAFs. Moreover, in tumor samples from 50 colon cancer patients, SNAI1 expression was associated with expression of other CAF markers, such as α-SMA and fibroblast activation protein. Interestingly, coculture of CAFs with colon cells induced a significant increase in epithelial cell migration and proliferation, which was associated with endogenous SNAI1 expression levels. Ectopic manipulation of Snail1 in fibroblasts demonstrated that Snail1 expression controlled migration as well as proliferation of cocultured colon cancer cells in a paracrine manner. Furthermore, expression of Snail1 in fibroblasts was required for the coadjuvant effect of these cells on colon cancer cell growth and invasion when coxenografted in nude mice. Finally, cytokine profile changes, particularly MCP-3 expression, in fibroblasts are put forward as mediators of Snail1-derived effects on colon tumor cell migration. In summary, these studies demonstrate that Snail1 is necessary for the protumorigenic effects of fibroblasts on colon cancer cells.es_ES
dc.description.sponsorshipThis research was supported by the PI12/02037, Fundación Científica AECC, SAF2010-20750, S2010/BMD-2344, RTICC-RD12/0036/0041 and by the Fundación Banco Santander. Antonio García de Herreros’ laboratory was supported by RTICC-RD12/0036/0005 and SAF 2010-16089. Ma Jesús Larriba’s laboratory was supported by RD12/0036/0021. Cristina Peña and José Miguel García are recipients of Miguel Servet Contracts from the Instituto de Salud Carlos III.es_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectCancer-associated fibroblastses_ES
dc.subjectColon canceres_ES
dc.subjectCell migrationes_ES
dc.titleProtumorigenic effects of Snail-expression fibroblasts on colon cancer cellses_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderComunidad de Madrid-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderFundación Científica Asociación Española Contra el Cáncer-
dc.contributor.funderBanco Santander-
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