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Title

Genetic responses in roots of a woody species upon colonization by a biocontrol endophytic bacterium: the olive-Pseudomonas fluorescens PICF7 case

AuthorsSchilirò, Elisabetta ; Ferrara, Massimo; Nigro, Franco; Mercado-Blanco, Jesús
Issue DateMay-2012
PublisherNew Phytologist Trust
Citation28th New Phytologist Symposium (2012)
AbstractInformation concerning processes underlying the interaction between beneficial bacteria and woody plants is still very limited and totally absent in the case of olive (Olea europaeaL.). We aimed to elucidate genomic responses occurring during the colonization of olive roots by the indigenous endophyte Pseudomonas fluorescens PICF7, an effective biocontrol agent against Verticillium wilt of olive (VWO). A Suppression Subtractive Hybridization cDNA library, corresponding to up-regulated genes, was generated from roots of olive plants (cv. Arbequina) inoculated with strain PICF7, enabling the identification of some 450 EST sequences differentially expressed during this interaction. Computational analysis showed a number of transcripts involved, among other processes, in plant defence response to different stresses (i.e., PAL, acetone cyanohydrin lyase, lipoxygenase, β-1,3-glucanase, endochitinases or lignin-forming peroxidases). Similarly, diverse transcription factors such as bHLH, GRAS1, ARF2 and SA-responsive WRKYs were shown to be induced in olive roots after PICF7 inoculation. Interestingly, 19% of detected olive transcripts corresponded to unidentified genes. Real-time quantitative PCR confirmed the induction of some of the aforementioned genes. Thus, the beneficial endophytic strain P. fluorescens PICF7 seems to trigger a broad array of defence responses in olive root tissues which can be responsible of its VWO biocontrol activity.
DescriptionPóster presentado en el 28th New Phytologist Symposium. Functions and Ecology of the Plant Microbiome, celebrado en Rodas (Grecia) del 18 al 21 de mayo de 2012.
URIhttp://hdl.handle.net/10261/97204
Appears in Collections:(IAS) Comunicaciones congresos
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