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Base Pairing Interaction between 5′- and 3′-UTRs Controls icaR mRNA Translation in Staphylococcus aureus

AuthorsRuiz de los Mozos, Igor ; Vergara-Irigaray, Marta ; Villanueva, Maite ; Bitarte, Nerea; Valle Turrillas, Jaione ; Solano Goñi, Cristina ; Lasa, Íñigo ; Toledo-Arana, Alejandro
Issue Date19-Dec-2013
PublisherPublic Library of Science
CitationPLoS Genetics 9(12): e1004001 (2013)
AbstractThe presence of regulatory sequences in the 3′ untranslated region (3′-UTR) of eukaryotic mRNAs controlling RNA stability and translation efficiency is widely recognized. In contrast, the relevance of 3′-UTRs in bacterial mRNA functionality has been disregarded. Here, we report evidences showing that around one-third of the mapped mRNAs of the major human pathogen Staphylococcus aureus carry 3′-UTRs longer than 100-nt and thus, potential regulatory functions. We selected the long 3′-UTR of icaR, which codes for the repressor of the main exopolysaccharidic compound of the S. aureus biofilm matrix, to evaluate the role that 3′-UTRs may play in controlling mRNA expression. We showed that base pairing between the 3′-UTR and the Shine-Dalgarno (SD) region of icaR mRNA interferes with the translation initiation complex and generates a double-stranded substrate for RNase III. Deletion or substitution of the motif (UCCCCUG) within icaR 3′-UTR was sufficient to abolish this interaction and resulted in the accumulation of IcaR repressor and inhibition of biofilm development. Our findings provide a singular example of a new potential post-transcriptional regulatory mechanism to modulate bacterial gene expression through the interaction of a 3′-UTR with the 5′-UTR of the same mRNA. © 2013 Ruiz de los Mozos et al.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pgen.1004001
Identifiersdoi: 10.1371/journal.pgen.1004001
issn: 1553-7390
e-issn: 1553-7404
Appears in Collections:(IDAB) Artículos
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