Please use this identifier to cite or link to this item:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

A sensitive method for confocal fluorescence microscopic visualization of starch granules in iodine stained samples

AuthorsOvecka, Miroslav CSIC; Bahaji, Abdellatif CSIC ORCID ; Muñoz Pérez, Francisco José CSIC ORCID ; Almagro, Goizeder CSIC ORCID ; Ezquer, Ignacio CSIC ORCID; Baroja-Fernández, Edurne CSIC ORCID CVN ; Li, Jun CSIC; Pozueta Romero, Javier CSIC ORCID
Issue Date2012
PublisherLandes Bioscience
CitationPlant Signaling and Behavior 7(9): 1146-1150 (2012)
AbstractSynthesized by glycogen synthase and starch synthases (SS) using ADP-glucose as the sugar donor molecule, glycogen and starch accumulate as predominant storage carbohydrates in most bacteria and plants, respectively. We have recently shown that the so-called >starch-less> Arabidopsis thaliana adg1-1 and aps1 mutants impaired in ADP-glucose pyrophosphorylase do indeed accumulate low starch content in normal growth conditions, and relatively high starch content when plants were cultured in the presence of microbial volatiles. Our results were strongly supported by data obtained using a highly sensitive method for confocal fluorescence microscopic visualization of iodine stained starch granules. Using Arabidopsis leaves from WT plants, aps1 plants, ss3/ss4 plants lacking both class III and class IV SS, gbss plants lacking the granule-bound SS, and sus1/sus2/sus3/sus4 plants lacking four genes that code for proteins with sucrose synthase activity, in this work we precisely describe the method for preparation of plant samples for starch microscopic examination. Furthermore, we show that this method can be used to visualize glycogen in bacteria, and pure starch granules, amylose and amylopectin. © 2012 Landes Bioscience.
Identifiersdoi: 10.4161/psb.21370
issn: 1559-2316
e-issn: 1559-2324
Appears in Collections:(IDAB) Artículos

Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

PubMed Central

checked on Jan 22, 2022


checked on Jan 17, 2022

Page view(s)

checked on Jan 22, 2022


checked on Jan 22, 2022

Google ScholarTM




Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.