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Título

Tandem amplification of a 28-kilobase region from the Yersinia enterocolitica chromosome containing the blaA gene

AutorSeoane, Asunción ; Sánchez, Emilia; García-Lobo, Juan M.
Fecha de publicaciónfeb-2003
EditorAmerican Society for Microbiology
CitaciónAntimicrob. Agents Chemother. 47(2) 682-688 (2003)
ResumenMost Yersinia enterocolitica strains are resistant to -lactam antibiotics due to the production of one or two chromosomally encoded -lactamases. Strain Y56 is a Y. enterocolitica O:3 serotype natural isolate that is resistant to moderate amounts of penicillins and that produces a single class A -lactamase. To select mutants with increased levels of resistance to -lactam antibiotics, strain Y56 was grown on plates containing increasing amounts of ampicillin, and variants resistant to up to 500 g of ampicillin per ml were obtained. Chromosomal DNA from hyperresistant isolates was analyzed by Southern hybridization with a blaA-specific probe to detect gene rearrangements. The use of pulsed-field gel electrophoresis revealed that the increase in the resistance level correlated with the amplification in tandem of a DNA fragment of about 28 kb containing the blaA gene. The phenotype of these isolates was not stable, and they recovered the basal low resistance level when the ampicillin used for selection was withdrawn from the growth medium. This loss of resistance was followed by the recovery of the original chromosomal structure. To understand this amplification process, the 28-kb amplification unit was cloned, and the ends were sequenced. The analysis of these sequences did not reveal the presence of either repeats or transposable elements to explain this process. However, we found short sequences similar to some DNA gyrase target sequences that have been described. In addition, we observed that the frequency of appearance of ampicillin-hyperresistant isolates by amplification of the blaA locus was lowered in the presence of the gyrase inhibitor novobiocin. These findings suggest that the DNA gyrase could be involved in this amplification event
Versión del editorhttp://aac.asm.org/content/47/2/682
URIhttp://hdl.handle.net/10261/97107
DOI10.1128/AAC.47.2.682-688.2003.
ISSN0066-4804
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