English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/97054
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants

AuthorsSerrano, Amaya ; Williams, Trevor G.; Simón, Oihane ; López-Ferber, Miguel; Caballero, Primitivo ; Muñoz, Delia
Issue Date2013
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 79: 1118-1125 (2013)
AbstractA natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. Both isolates comprise a high proportion of large-deletion genotypes that lack genes that are essential for viral replication or transmission. To determine the likely origins of such genotypically similar population structures, we performed genomic and functional analyses of these genotypes. The homology of nucleotides in the deleted regions was as high as 79%, similar to those of other colinear genomic regions, although some SfMNPV genes were not present in SeMNPV. In addition, no potential consensus sequences were shared between the deletion flanking sequences. These results indicate an evolutionary mechanism that independently generates and sustains deletion mutants within each virus population. Functional analyses using different proportions of complete and deletion genotypes were performed with the two viruses in mixtures of occlusion bodies (OBs) or co-occluded virions. Ratios greater than 3:1 of complete/deletion genotypes resulted in reduced pathogenicity (expressed as median lethal dose), but there were no significant changes in the speed of kill. In contrast, OB yields increased only in the 1:1 mixture. The three phenotypic traits analyzed provide a broader picture of the functional significance of the most extensively deleted SeMNPV genotype and contribute toward the elucidation of the role of such mutants in baculovirus populations. © 2013, American Society for Microbiology.
URIhttp://hdl.handle.net/10261/97054
DOIhttp://dx.doi.org/10.1128/AEM.03021-12
Identifiersdoi: 10.1128/AEM.03021-12
issn: 0099-2240
e-issn: 1098-5336
Appears in Collections:(IDAB) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.