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dc.contributor.authorAragón, Juan J.-
dc.contributor.authorSánchez Cela, Vicente-
dc.contributor.authorBoto, Luis-
dc.date.accessioned2009-01-19T12:13:57Z-
dc.date.available2009-01-19T12:13:57Z-
dc.date.issued1986-
dc.identifier.citationEuropean Journal of Biochemistry 161(3):757-761 (1986)en_US
dc.identifier.urihttp://hdl.handle.net/10261/9698-
dc.description.abstractThe occurrence of fructose 2,6-bisphosphate was detected in Dictyostelium discoideum. The levels of this compound were compared with those of cyclic AMP and several glycolytic intermediates during the early stages of development. Removal of the growth medium and resuspension of the organism in the differentiation medium decreased the content of fructose 2,6-bisphosphate to about 20% within 1 h, remaining low when starvationinduced development was followed for 8 h. The content of cyclic AMP exhibited a transient increase that did not correlate with the change in fructose 2,dbisphosphate. If after 1 h of development 2% glucose was added to the differentiation medium, fructose 2,6-bisphosphate rapidly rose to similar levels to those found in the vegetative state, while the increase in cyclic AMP was prevented. The contents of hexose 6-phosphates, fructose 1,6-bisphosphate and triose phosphates changed in a way that was parallel to that of fructose 2,6-bisphosphate, and addition of sugar resulted in a large increase in the levels of these metabolites. The content of fructose 2,6-bisphosphate was not significantly modified by the addition of the 8-bromo or dibutyryl derivatives of cyclic AMP to the differentiation medium. These results provide evidence that the changes in fructose 2,6-bisphosphate levels in D. discoideum development are not related to a cyclic-AMP-dependent mechanism but to the availability of substrate. Fructose 2,6-bisphosphate was found to inhibit fiuctose-l,6-bisphosphatasaec tivity of this organism at nanomolar concentrations, while it does not affect the activity of phosphofructokinase in the micromolar range. The possible physiological implications of these phenomena are discussed.en_US
dc.description.sponsorshipComisión Asesora para la Investigación Científica y Técnicaen_US
dc.format.extent543956 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherWiley-Blackwellen_US
dc.rightsclosedAccessen_US
dc.titleFructose 2,6-bisphosphate in Dictyostelium discoideum.Independence of cyclic AMP production and inhibition of fructose-1,6-bisphosphataseen_US
dc.typeArtículoen_US
dc.identifier.doi10.1111/j.1432-1033.1986.tb10504.x-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1111/j.1432-1033.1986.tb10504.x-
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