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Proteomic mapping of the lung vascular endothelial cell surface in Schistosoma bovis-infected hamsters

AutorTorre Escudero, Eduardo de la ; Pérez Sánchez, Ricardo ; Manzano Román, Raúl ; Oleaga, Ana
Palabras claveSchistosoma bovis
Pulmonary vasculature
Fecha de publicación2014
CitaciónJournal of Proteomics 106: 86–98 (2014)
ResumenSchistosomes are blood trematodes that are perfectly adapted to living in their intravascular habitat and to achieve this they have developed mechanisms enabling them to evade the immune and haemostatic responses of the host and to regulate endothelial cell function to favour their own survival. The objective of this work was to analyse the changes induced by Schistosoma bovis schistosomula in the proteome expressed by infected hamsters, over 10 and 20 days, on the endothelial surface of their pulmonary vasculature. To accomplish this, we subjected the lungs of non-infected and S. bovis-infected hamsters to vascular perfusion with a biotin ester reactive. Analysis by liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) of endothelial surface proteins resulted in the identification of a total of 459 non-redundant proteins in the lung vasculature of infected and non-infected hamsters. Here we report the proteins identified, classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. bovis-infected hamsters for ten and twenty days. This work provides the first data on the vascular surface proteome of the lung after S. bovis infection and identifies some of the changes induced in it during infection.
Descripción46 páginas, 6 figuras, 3 tablas.--The definitive version is available at http://www.elsevier.com
Versión del editorhttp://dx.doi.org/10.1016/j.jprot.2014.04.025
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