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Lasting effects of early temperature on European sea bass (Dicentrarchux labrax) sex differentiation: Endocrinology meets epigenetics

AutorDíaz, Noelia ; Piferrer, Francesc
Fecha de publicaciónjul-2013
Citación17th ICCE (2013)
ResumenThe European sea bass is a gonochoristic teleost with a polygenic sex determination system influenced by temperature. High temperatures masculinize genetic females, hence skewing sex ratios to ~3:1 in favour of males. Recently, we showed that the underlying mechanism includes hypermethylation of the gonadal aromatase (cyp19a1) promoter, linking early environment with subsequent juvenile and adult sex ratios. However, the effects of this early temperature at the time of sex differentiation are unknown. In the present study, European sea bass larvae were exposed to either natural (15–17°C; NT) or artificially high (21°C; HT) temperature from 20 to 90 days post-fertilization (dph), comprising the period of early gonad formation. Sex differentiating gonads were analysed at 170 dph at the whole transcriptome level by a custom-made microarray. As expected, HT masculinized genetic females, with twice as males as in the NT group. Transcriptomic analysis showed that 34 genes were differentially expressed (21 up- and 13 down- regulated genes) in the HT group, including epigenetic maintainers such as hdac11 and these results were confirmed by qPCR. Analysis of sex differentiation-related genes showed an increase in the expression of pro-male genes (amh, dmrt1) and a decrease in the expression of pro-female genes (cyp19a1) at HT supporting the observed masculinization. Also, some genes related to epigenetic modifications (pgrfg2) were up-regulated in the HT group, but the meaning of these changes needs further research. This study contributes to our understanding of how the early environment sets permanent changes that result in long-lasting consequences, in this case in the sexual phenotype. It also combines the behaviour of cognate genes related to sex differentiation with that of genes involved in maintaining cell identity through epigenetic mechanisms. This interaction opens a new perspective in our understanding of reproductive endocrinology
Descripción17th International Congress of Comparative Endocrinology (ICCE), 15-19 July 2013, Barcelona, Spain
Versión del editorhttp://www.icce2013.com/USB/authors.html
Aparece en las colecciones: (ICM) Comunicaciones congresos
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