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Título

Effects of temperature and Estradiol administration on the whole transcriptome of European sea bass (Dicentrarchus labrax) gonads

Autor Díaz, Noelia ; Piferrer, Francesc
Fecha de publicación 14-jul-2013
Citación 9th Congress of the Asociación Ibérica de Endocrinología Comparada. Final Program - Abstracts Book: 36 (2013)
ResumenThe European sea bass is a gonochoristic teleost with a polygenic sex determination system influenced by temperature. High temperatures masculinize genetic females, hence skewing sex ratios to ~3:1 in favor of males. In the present study, sea bass larvae were exposed either to natural (NT: 17ºC) or to artificially high temperature (HT: 21ºC) from 20 to 90 days post-hatch (dph), comprising the period of early gonad formation. Then during the hormone sensitive period (90–160 dph) three groups of fish were treated either with ethanol- (NT and HT) or with Estradiol (E2)-sprayed food (HTE2). As expected, high temperature masculinized genetic females (from 40% males at NT to 78% males at HT) and the E2 treatment resulted in an all-female population (0% males). A custom-made microarray (MA) was used to determine profiles of gene expression in the gonads of putative females at 170 dph, which were identified by cluster analysis as the individuals with the highest gonadal aromatase (cyp19a) expression, a marker of female differentiation. Transcriptomic analysis of HTE2 vs. HT showed 420 differentially expressed (DE) genes due to E2 treatment (100 up- and 320 down-regulated genes), including 27 DE genes (12 up- and 15 down-regulated genes) solely due to temperature. qPCR-validated MA results showed an increase in the expression of pro-male genes such as amh, dmrt1 or STAR in HT vs. NT, supporting the observed masculinization by heat. Also, a decrease in the expression of these same genes at HTE2 vs. HT was observed as a consequence of the feminization by E2. Together these results corroborate the connection between elevated temperature and the resulting sex ratios. Further analysis of altered gene expression levels will help to understand the pathways affected, by both temperature and E2
Descripción 9th Congress of the Asociación Ibérica de Endocrinología Comparada (AIEC), 13-14 July 2013, Barcelona, Spain
URI http://hdl.handle.net/10261/96770
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