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Title

Communication between L-galactono-1,4-lactone dehydrogenase and cytochrome c

AuthorsHervás, Manuel CSIC ORCID; Bashir, Qamar; Leferink, Nicole G.H.; Ferreira, Patricia CSIC ORCID; Moreno-Beltrán, Blas CSIC ORCID; Westphal, Adrie H.; Díaz-Moreno, Irene CSIC ORCID; Medina, Milagros; Rosa, Miguel A. de la CSIC ORCID; Ubbink, Marcellus; Navarro, José A. CSIC ORCID ; van Berkel, Willem, J.H.
KeywordsCytochrome c
Electron transfer
Flavoprotein
Protein-protein interaction
Vitamin c
Issue Date2013
PublisherJohn Wiley & Sons
CitationFEBS Journal, 280(8): 1830-1840 (2013)
Abstractl-galactono-1,4-lactone dehydrogenase (GALDH) catalyzes the terminal step of vitamin C biosynthesis in plant mitochondria. Here we investigated the communication between Arabidopsis thaliana GALDH and its natural electron acceptor cytochrome c (Cc). Using laser-generated radicals we observed the formation and stabilization of the GALDH semiquinone anionic species (GALDHSQ). GALDHSQ oxidation by Cc exhibited a nonlinear dependence on Cc concentration consistent with a kinetic mechanism involving protein–partner association to form a transient bimolecular complex prior to the electron transfer step. Oxidation of GALDHSQ by Cc was significantly impaired at high ionic strength, revealing the existence of attractive charge–charge interactions between the two reactants. Isothermal titration calorimetry showed that GALDH weakly interacts with both oxidized and reduced Cc. Chemical shift perturbations for 1H and 15N nuclei of Cc, arising from the interactions with unlabeled GALDH, were used to map the interacting surface of Cc. For Arabidopsis Cc and yeast Cc, similar residues are involved in the interaction with GALDH. These residues are confined to a single surface surrounding the heme edge. The range of chemical shift perturbations for the physiological Arabidopsis Cc–GALDH complex is larger than that of the non-physiological yeast Cc–GALDH complex, indicating that the former complex is more specific. In summary, the results point to a relatively low affinity GALDH–Cc interaction, similar for all partner redox states, involving protein–protein dynamic motions. Evidence is also provided that Cc utilizes a conserved surface surrounding the heme edge for the interaction with GALDH and other redox partners.
URIhttp://hdl.handle.net/10261/96769
DOI10.1111/febs.12207
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