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Title: | Decolorization of synthetic dyes by laccase immobilized on epoxy-activated carriers |
Authors: | Kunamneni, Adinarayana ![]() ![]() ![]() ![]() ![]() ![]() |
Keywords: | Covalent immobilization Epoxy Carriers Acrylic polymers Synthetic dyes Biocatalysis Biotransformation |
Issue Date: | Feb-2008 |
Publisher: | Elsevier |
Citation: | Process Biochemistry 43(2): 169-178 (2008) |
Abstract: | The Myceliophthora thermophila laccase was covalently immobilized on polymethacrylate-based polymers (Sepabeads EC-EP3 and Dilbeads NK) activated with epoxy groups. The enzyme immobilized on Sepabeads EC-EP3 exhibited notable activity (203 U/g) along with remarkably improved stability towards pH, temperature and storage time, but no increased resistance to organic solvents. In addition, the immobilized laccase also showed good operational stability, maintaining 84% of its initial activity after 17 cycles of oxidation of ABTS. The immobilized biocatalyst was applied to the decolorization of six synthetic dyes. Immobilized laccase retained 41% activity in the decolorization of Methyl Green in a fixed-bed reactor after five cycles. The features of these biocatalysts are very attractive for their application on the decolorization of dyes in the textile industry in batch and continuous fixed-bed bioreactors. To our knowledge, this is the first report on immobilization of laccase on Sepabeads carriers and its efficient dyes decolorization. |
Publisher version (URL): | http://doi.org/10.1016/j.procbio.2007.11.009 |
URI: | http://hdl.handle.net/10261/9672 |
DOI: | http://dx.doi.org/10.1016/j.procbio.2007.11.009 |
ISSN: | 1359-5113 |
E-ISSN: | 1873-3298 |
Appears in Collections: | (ICP) Artículos (CIB) Artículos |
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postprint-process biochemistry- decolorization.pdf | 2,86 MB | Adobe PDF | ![]() View/Open |
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