English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/96711
Compartir / Impacto:
Add this article to your Mendeley library MendeleyBASE
Citado 18 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)

Immunolocalization of antioxidant enzymes in high-pressure frozen root and stem nodules of Sesbania rostrata

Autor Rubio Luna, María Carmen ; Becana Ausejo, Manuel ; Kanematsu, Sumio; Ushimaru, Takashi; James, Euan Kevin
Palabras clave Photosynthesis
Nitrogen fixation
Reactive oxygen species (ROS)
Sesbania rostrata
Vascular tissue
Superoxide dismutase (SOD)
Fecha de publicación jul-2009
EditorBlackwell Publishing
Citación New Phytologist 183 (2): 395-407 (2009)
ResumenThe activities and localizations of superoxide dismutases (SODs) were compared in root and stem nodules of the semi-aquatic legume Sesbania rostrata using gel-activity assays and immunogold labelling, respectively. Nodules were fixed by high-pressure freezing and dehydrated by freeze substitution. Stem nodules showed more total and specific SOD activities than root nodules because of the presence of chloroplastic CuZnSOD. Most of the total SOD activity of stem and root nodules resulted from 'cytosolic' CuZnSOD, localized in the cytoplasm and chromatin, and from MnSOD in the bacteroids and in the mitochondria of vascular tissue. FeSOD was present in nodule plastids and in leaf chloroplasts, and was found to be associated with chromatin. Superoxide production was detected histochemically in the vascular bundles and in the infected tissue of stem and root nodules, whereas peroxide accumulation was observed in the cortical cell walls and intercellular spaces, as well as within the infection threads of both nodule types. These data suggest a role of CuZnSOD and FeSOD in protecting nuclear DNA from reactive oxygen species and/or in modulating gene activity. The enhanced levels of CuZnSOD, MnSOD and superoxide production in vascular bundle cells are consistent with a role of CuZnSOD and superoxide in the lignification of xylem vessels, but also suggest additional functions in coping with superoxide production by the high respiratory activity of parenchyma cells.
Descripción 34 Pags.- 7 Figs.- 1 Tabl. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1469-8137
Versión del editorhttp://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2009.02866.x/abstract
URI http://hdl.handle.net/10261/96711
Identificadoresdoi: 10.1111/j.1469-8137.2009.02866.x
issn: 0028-646X
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
BecanaM_NewPhytol_2009.pdf38,27 MBAdobe PDFVista previa
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.