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Immunolocalization of antioxidant enzymes in high-pressure frozen root and stem nodules of Sesbania rostrata

AuthorsRubio Luna, María Carmen ; Becana Ausejo, Manuel ; Kanematsu, Sumio; Ushimaru, Takashi; James, Euan Kevin
Nitrogen fixation
Reactive oxygen species (ROS)
Sesbania rostrata
Vascular tissue
Superoxide dismutase (SOD)
Issue DateJul-2009
PublisherBlackwell Publishing
CitationNew Phytologist 183 (2): 395-407 (2009)
AbstractThe activities and localizations of superoxide dismutases (SODs) were compared in root and stem nodules of the semi-aquatic legume Sesbania rostrata using gel-activity assays and immunogold labelling, respectively. Nodules were fixed by high-pressure freezing and dehydrated by freeze substitution. Stem nodules showed more total and specific SOD activities than root nodules because of the presence of chloroplastic CuZnSOD. Most of the total SOD activity of stem and root nodules resulted from 'cytosolic' CuZnSOD, localized in the cytoplasm and chromatin, and from MnSOD in the bacteroids and in the mitochondria of vascular tissue. FeSOD was present in nodule plastids and in leaf chloroplasts, and was found to be associated with chromatin. Superoxide production was detected histochemically in the vascular bundles and in the infected tissue of stem and root nodules, whereas peroxide accumulation was observed in the cortical cell walls and intercellular spaces, as well as within the infection threads of both nodule types. These data suggest a role of CuZnSOD and FeSOD in protecting nuclear DNA from reactive oxygen species and/or in modulating gene activity. The enhanced levels of CuZnSOD, MnSOD and superoxide production in vascular bundle cells are consistent with a role of CuZnSOD and superoxide in the lignification of xylem vessels, but also suggest additional functions in coping with superoxide production by the high respiratory activity of parenchyma cells.
Description34 Pags.- 7 Figs.- 1 Tabl. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1469-8137
Publisher version (URL)http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2009.02866.x/abstract
Identifiersdoi: 10.1111/j.1469-8137.2009.02866.x
issn: 0028-646X
Appears in Collections:(EEAD) Artículos
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