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Chromosome genomics facilitates mapping and sequencing the complex genome of bread wheat

AutorDoležel, Jaroslav; Hernández Molina, Pilar ; Kilian, Andrzej
Fecha de publicaciónoct-2011
EditorCzech Society for Analytical Cytology
CitaciónAnalytical Cytometry VI Conference (2011)
ResumenBread wheat is one of the most important crops, providing g|obally 20% of allfood caIoríes' ln order to satisfy the increasing demand by the growing population, new varieties are needed with increased yield and resistance to bioťc and abitotic stress' Molecular tools can support the breeding process, but their development in wheat has been held back by its large genome (-17 Gbp), comprising three related (homoeologous) genomes and majority of repeťťve DNA. These features hamper genetic mapping, positional cloning and genome sequencing. While the advances in sequencíng technology make it possible to sequence the wheat genome, any such effort willface difficulťes to assemble the huge number of sequence reads. One solution is to reduce the genome complexity by dissecting the genome to small fractions by flow-sorťng individual chromosomes and chromosome arms' Recently, chromosome genomics has been also established in wild relaťves of wheat from genus Aegilops and should aid in introducing new alleles and genes to cultivated wheat. Chromosome genomics in wheat encompasses a large portfolio of applications, including the construction of ch romosome-specific BAC libraries, assembly of physical maps, high-throughput physical mapping on DNA microarrays and targeted development of molecular markers from specific chromosomes and chromosome arms. Sequencing of chromosomal DNA as well as clones from ch romosome-specific BAC libraries using the next generation sequencíng technologies is a rapid|y expanding application with a great potentíal as it enables to tackle the genome in a step-wise manner, to resolve problems relating to polyploidy, to perform comparaťve chromosomal studies and to subdivide the work on sequencing the huge wheat genome among various laboratories.
DescripciónPóster presentado en la Analytical Cytometry VI Conference, celebrada en Praga del 8 al 11 de octubre de 2011. Doležel, Jaroslav et al.
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