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Título

Electrochemical biosensing of pesticide residues based on affinity biocomposite platforms.

Autor Zacco, E.; Galvé, Roger; Marco, María Pilar; Alegret, Salvador; Pividori, María Isabel
Palabras clave Graphite-epoxy biocomposite
Protein A
Avidin
Atrazine
Electrochemical biosensing
Immunoassay
Fecha de publicación 15-mar-2007
EditorElsevier
Citación Biosensors & Bioelectronics 22(8): 1707-1715 (2007)
ResumenA novel and very sensitive electrochemical immunosensing strategy for the detection of atrazine based on affinity biocomposite transducers is presented. Firstly, the graphite–epoxy composite transducer was bulk-modified with different universal affinity biomolecules, such as avidin and Protein A. Two strategies for the immobilization of the anti-atrazine antibodies on both biocomposite transducers were evaluated: ‘wet-affinity’ and ‘dry-assisted affinity’ immobilization. Finally, the performance of a novel anti-atrazine immunocomposite bulk-modified with anti-atrazine antibodies was also evaluated. The better immobilization performance of the anti-atrazine antibodies was achieved by ‘dry-assisted affinity’ immobilization on Protein A (2%) graphite–epoxy biocomposite (ProtA(2%)-GEB) as a transducer. The immunological reaction for the detection of atrazine performed on the ProtA(2%)-GEB biosensors is based on a direct competitive assay using atrazine-HRP tracer as the enzymatic label. The electrochemical detection is thus achieved through a suitable substrate and a mediator for the enzyme HRP. This novel strategy was successfully evaluated using spiked orange juice samples. The detection limit for atrazine in orange juices using the competitive electrochemical immunosensing assay was found to be 6×10−3 μgL−1 (0.03 nmol L−1) thus this biosensing method accomplishes by far the LODs required for the European Community directives for potable water and food samples (0.1 μgL−1). This strategy offers great promise for rapid, simple, cost effective, and on-site biosensing of biological, food, and environmental samples.
Descripción 9 pages, 5 figures.-- PMID: 17085033 [PubMed].
Available online on Nov 7, 2006.
Versión del editorhttp://dx.doi.org/10.1016/j.bios.2006.07.037
URI http://hdl.handle.net/10261/9630
DOI10.1016/j.bios.2006.07.037
ISSN0956-563
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