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Título

Surface-enhanced resonance Raman scattering spectroscopy of bacterial photosynthetic membranes: orientation of the carotenoids of Rhodobacter sphaeroides 2.4.1

Autor Picorel Castaño, Rafael ; Lu, Tianhong; Holt, Randall E.; Cotton, Therese M.; Seibert, Michael
Palabras clave Cell Membrane
Carotenoids
Bacterial Chromatophores
Rhodobacter sphaeroides
Photosynthetic membrane
Photosynthesis
Spectrum Analysis
Raman spectrometry
Membrane structure
Fecha de publicación 1990
EditorAmerican Chemical Society
Citación Biochemistry 29 (3): 707-712 (1990)
ResumenSurface-enhanced resonance Raman scattering (SERRS) spectra were obtained from carotenoids, in the all-trans configuration, located on the antenna complexes of Rhodobacter sphaeroides 2.4.1 membranes. Since resonance Raman (RR) spectra are barely detectable at the concentration that SERRS signals saturate, SERRS represents a very sensitive means of detecting pigments in biological systems. Prominent SERRS spectra of sphaeroidenone were detected in chromatophores (cytoplasmic side out) but not in spheroplast-derived vesicles (periplasmic side out), demonstrating that the carotenoid is asymmetrically located on the cytoplasmic side of the cell membrane. Comparison of peak frequencies from SERRS and RR spectral data suggests that the carotenoids are oriented into the membrane with the methoxy end of the isoprenoid chains located closest to the cytoplasmic side of the intracytoplasmic membrane. This work not only shows that SERRS spectroscopy can provide information on the location of a chromophore in a biological membrane but also for the first time demonstrates that SERRS data can be used to ascertain the orientation of a chromophore within the membrane. This observation greatly increases the potential of this technique for structural analysis of intact membranes at the molecular level. © 1990 American Chemical Society.
Versión del editorhttp://pubs.acs.org/doi/abs/10.1021/bi00455a017
URI http://hdl.handle.net/10261/96202
DOI10.1021/bi00455a017
Identificadoresdoi: 10.1021/bi00455a017
issn: 0006-2960
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