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Application of cDNA-AFLP approach to study pea resistance to Didymella pinodes in Pisum fulvum at molecular level

AuthorsRispail, Nicolas ; Fondevilla, Sara ; Rubiales, Diego
Issue DateMay-2013
PublisherInternational Legume Society
CitationFirst Legume Society Conference (2013)
AbstractAscochyta blight is one of the most important funga l diseases of pea worldwide. This disease is caused by a complex of fungal pathogens including Didymella pinodes, Phoma medicaginis and Ascochyta pisi . Among these, D. pinodes is the most predominant and damaging. So far, no complete resistance to this pathogen has been descri bed in pea. The highest levels of incomplete resistance have been identified in the wild relative o f pea Pisum fulvum . However, the genes involved in the defence reaction to D. pinodes in this species are still unknown. Thus, a cDNA2 AFLP approach has been undertaken to compare gene ex pression pattern between the susceptible P. sativum cv. Messire and the highly resistant P. fulvum P651 accessions. After digestion of RNA samples with EcoR I and Mse I restriction enzymes, the specific amplification using AFLP primer with 1 and 3 selective nucleotides respectively allowed to detect a mean of 52 markers per primer combination. Interestingly, 48% of all markers detected were differentially expressed, varying mainly between genotype indicating a high degree of polymorphism between these accessions. In addition, 20% of the markers wer e differentially expressed between treatments. Identification and characterisation of th ese gene fragments is currently underway. This will allow identifying defensive genes involved s pecifically in the pathosystem pea2 D. pinodes and provide new molecular markers that will be very v aluable for future mapping projects.
DescriptionPóster presentado en la First Legume Society Conference, celebrada en Novi Sad (Serbia) del 9 al 11 de mayo de 2013.
Appears in Collections:(IAS) Comunicaciones congresos
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