English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/95878
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Different repair pathways are involved in checkpoint-dependent replication fork stabilization

AuthorsSegurado, Mónica; Diffley, John F. X.
Issue Date2006
CitationMini-Symposium RTN (2006)
AbstractThe ability to survive spontaneous and induced DNA damage depends on S phase checkpoints, which act by inhibiting late origin firing, stabilizing replication forks and preventing S phase progression. It has been previously shown in our lab that the preservation of fork integrity, rather than inhibition of origin firing, is essential to mantain cell viability under DNA damage conditions. Therefore, we are interested in the identification of checkpoint-dependent pathways involved in stabilization of the forks. We have initiated a genetic screen to identify mutants that render checkpoint-defective yeast cells more resistant to fork-stalling agents. In our first attempt we identified a number of mutants but we were unable to complement the mutants because of the partially penetrant phenotype. We have screened the entire deletion library for mutants with heightened resistance to hydroxyurea when the checkpoint has been compromised with caffeine. From this, we have identified mutants in several repair pathways, and therefore, we decided to check for caffeine plus hydroxyurea resistance in several members of the main repair pathways. Our conclusions indicate that different repair pathways contribute to lethality in checkpoint mutants.
DescriptionTrabajo presentado al Mini-Symposium RTN: Genome Integrity, celebrado en Paris del 11 al 12 de diciembre de 2006.
Appears in Collections:(IMB) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.