English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/95876
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Genome-wide nucleosome profile during mitosis and meiosis in fission yeast

AuthorsSoriano, Ignacio ; Marín, Laura ; Quintales, Luis ; Antequera, Francisco
Issue Date2011
CitationSixth International Fission Yeast Meeting: pombe 2011
AbstractThe eukaryotic genome is packaged in the nucleus as chromatin, whose fundamental units are nucleosomes. To study the role of nucleosome organization in the regulation of transcription, replication and recombination, we have generated genome-wide, highresolution maps of nucleosomes in Schizosaccharomyces pombe during mitosis and meiosis using tiling microarrays and next-generation sequencing. Our results show that most of the genome is occupied by positioned nucleosomes and that this pattern is largely conserved in mitosis and meiosis. We have identified approximately 3000 nucleosome-depleted regions (NDRs), of which 75% are identical in mitosis and meiosis. Global transcription analyses revealed local remodeling of the promoters of genes expressed at specific times during meiosis. We also found that high rates of transcription and antisense RNAs caused the delocalization of nucleosomes along the transcribed regions. Large intergenic regions (IGRs) often included clusters of nucleosome-depleted regions (NDRs), many of which colocalized with sites of double-strand breaks during meiosis. By contrast, the binding of the Origin Recognition Complex to A+T-rich replication origins did not colocalize with NDRs. Furthermore, poly (dA:dT) tracts displayed a tendency to map between nucleosomes but did not generate extensive NDRs. Together, our results show that the nucleosome organization of the S. pombe genome is to a large extent constitutive, and that very local remodeling takes place in response to the activation of the specific transcriptional programs during mitosis and meiosis.
DescriptionTrabajo presentado al Sixth International Fission Yeast Meeting celebrado en Boston (USA) del 25 al 30 de junio de 2011.
URIhttp://hdl.handle.net/10261/95876
Appears in Collections:(IBFG) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
Genome-wide nucleosome.pdf1,24 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.