English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/95811
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Voltammetric study of the adsorbed thermophilic plastocyanin from Phormidium laminosum up to 90 °c

AuthorsOlloqui-Sariego, José Luis; Frutos-Beltrán, Estrella ; Roldán, Emilio; Rosa, Miguel A. de la ; Calvente, Juan José; Díaz-Quintana, Antonio; Andreu, Rafael
KeywordsRedoxthermodynamics
Plastocyanin
Proteinfilm voltammetry
Directelectrontransfer
Azurin
Proteinthermostability
Issue Date2012
PublisherElsevier
CitationElectrochemistry Communications 19: 105- 107 (2012)
AbstractRedox thermodynamics and kinetics of plastocyanin from Phormidium laminosum, and of azurin from Pseudonomas aeruginosa, have been investigated as a function of temperature by protein film voltammetry. To this purpose, both proteins have been physisorbed on a pyrolytic graphite edge electrode. A pronounced negative shift of the plastocyanin standard potential, compared to a slight shift in the case of azurin, has been found upon increasing the temperature. Hence, significant conformational and/or solvation changes accompany the redox conversion of plastocyanin. Lower electron transfer rate constants (by c.a. one order of magnitude) and higher activation enthalpies have been found for plastocyanin as compared to azurin. The voltammetric response of azurin vanishes irreversibly at temperatures close to 60 °C, whereas the redox properties of plastocyanin remain unaltered, except for some loss of electroactive protein, after heating the electrode at temperatures as high as 90 °C. © 2012 Elsevier B.V. All rights reserved.
URIhttp://hdl.handle.net/10261/95811
DOI10.1016/j.elecom.2012.03.012
Identifiersdoi: 10.1016/j.elecom.2012.03.012
issn: 1388-2481
Appears in Collections:(IBVF) Artículos
Files in This Item:
File Description SizeFormat 
1-s2.0-S1388248112001117-main.pdf343,27 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.