English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/9575
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Combining microsatellite markers and capillary gel electrophoresis with laser-induced fluorescence to identify the grape (Vitis vinifera) variety of musts

AutorRodríguez-Plaza, P.; González García, Ramón ; Moreno-Arribas, M. Victoria ; Polo, María Carmen; Bravo, Gema; Martínez-Zapater, José M. ; Martínez Rodríguez, María del Carmen ; Cifuentes, Alejandro ; Cifuentes, Alejandro
Palabras claveGrapevine characterization
Capillary gel electrophoresis
Polymerase chain reaction
Fecha de publicación31-ene-2006
CitaciónEuropean Food Research and Technology 223: 625–631 (2006)
ResumenIn this work, a new method that combines the use of microsatellite markers (VVMD5 and ZAG79) together with capillary gel electrophoresis with laser-induced fluorescence (CGE–LIF) is developed and applied to the identification of Albariño and Moscatel Grano Menudo musts. The GCE-LIF method uses commercially available products including polymers, DNA-intercalating dyes and bare fused silica capillaries to provide reproducible and sensitive separations of DNA fragments for grapevine characterization. The CGE–LIF procedure offers highly resolved separations of DNA fragments from 48 to 1031 bp in ca. 30 min with efficiencies up to 1.8×106 plates/m allowing the separation of fragments that differ in 4 bp. The use of different DNA standards (i.e., 100 bp ladder, Ф×174 and pBR322) and their effect on size assignment of the amplified DNA is also investigated. It is demonstrated that the microsatellite markers (VVMD5 and ZAG79) provide DNA amplification patterns specific for Albariño and Moscatel Grano Menudo grapes that can be adequately differentiated by using CGE–LIF. Moreover, the DNA sizes determined by this CGE–LIF method are corroborated using a more standard procedure (i.e., an automatic genetic analyzer with a commercial kit) demonstrating the usefulness of this new methodology.
Versión del editorhttp://dx.doi.org/10.1007/s00217-005-0244-2
ISSN1438-2377 (Print)
1438-2385 (Online)
Aparece en las colecciones: (IFI) Artículos
(MBG) Artículos
(CNB) Artículos
(ICVV) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.