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Monolignol ferulate transferase introduces chemically labile linkages into the lignin backbone

AuthorsWilkerson, C.G.; Mansfield, S.D.; Lu, Fachuang; Withers, S.; Park, J. Y.; Karlen, S.D.; Gonzales-Vigil, E.; Padmakshan, D.; Unda, F.; Rencoret, Jorge ; Ralph, John
Aromatic polymer
Plant cell walls
Chemical depolymerization
Industrial processing
Issue Date4-Apr-2014
PublisherAmerican Association for the Advancement of Science
CitationScience 344 (6179): 90-93 (2014
AbstractRedesigning lignin, the aromatic polymer fortifying plant cell walls, to be more amenable to chemical depolymerization can lower the energy required for industrial processing. We have engineered poplar trees to introduce ester linkages into the lignin polymer backbone by augmenting the monomer pool with monolignol ferulate conjugates. Herein, we describe the isolation of a transferase gene capable of forming these conjugates and its xylem-specific introduction into poplar. Enzyme kinetics, in planta expression, lignin structural analysis, and improved cell wall digestibility after mild alkaline pretreatment demonstrate that these trees produce the monolignol ferulate conjugates, export them to the wall, and use them during lignification. Tailoring plants to use such conjugates during cell wall biosynthesis is a promising way to produce plants that are designed for deconstruction.
Description4 páginas.-- 4 figuras.-- 1 tabla.-- 27 referncias.--- El pdf. del Material Suplementarío tiene 29 páginas. Contiene: Materials and Methods.-- Supplementary Text.-- Figs. S1 to S9.-- Tables S1 to S4.-- 39 References
Publisher version (URL)http://dx.doi.org/10.1126/science.1250161
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