English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/95562
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Autoinhibition of TBCB regulates EB1-mediated microtubule dynamics

AuthorsCarranza, Gerardo; Castaño, Raquel; Fanarraga, Mónica L.; Villegas, Juan Carlos; Gonçalves, Joao; Soares, Helena; Ávila, Jesús ; Marenchino, Marco; Campos-Olivas, Ramón; Montoya, Guillermo; Zabala, Juan Carlos
KeywordsMicrotubule dynamics
TIPs
Tubulin folding cofactors
CCT
Issue Date2013
PublisherSpringer
CitationCellular and Molecular Life Sciences 70: 357- 371 (2013)
AbstractTubulin cofactors (TBCs) participate in the folding, dimerization, and dissociation pathways of the tubulin dimer. Among them, TBCB and TBCE are two CAP-Gly domain-containing proteins that together efficiently interact with and dissociate the tubulin dimer. In the study reported here we showed that TBCB localizes at spindle and midzone microtubules during mitosis. Furthermore, the motif DEI/M-COO- present in TBCB, which is similar to the EEY/F-COO- element characteristic of EB proteins, CLIP-170, and α-tubulin, is required for TBCE-TBCB heterodimer formation and thus for tubulin dimer dissociation. This motif is responsible for TBCB autoinhibition, and our analysis suggests that TBCB is a monomer in solution. Mutants of TBCB lacking this motif are derepressed and induce microtubule depolymerization through an interaction with EB1 associated with microtubule tips. TBCB is also able to bind to the chaperonin complex CCT containing α-tubulin, suggesting that it could escort tubulin to facilitate its folding and dimerization, recycling or degradation. © 2012 Springer Basel AG.
URIhttp://hdl.handle.net/10261/95562
DOI10.1007/s00018-012-1114-2
Identifiersdoi: 10.1007/s00018-012-1114-2
issn: 1420-682X
Appears in Collections:(CBM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.