English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/95531
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Effect of PHA and LPS on the activity of nonspecific cytotoxic cells and macrophages of rainbow trout (Oncorhynchus mykiss)

Other TitlesEfecto del PHA y LPS en la actividad de las células citotóxicas naturales y macrófagos de trucha arcoíris (Oncorhynchus mykiss)
AuthorsGómez, Leonardo; Cortés, Raúl ; Smith, C. T.
KeywordsFasR-FasL
Lectins
Leukocytes
Innate immunity
Interleukins
Issue Date2013
PublisherUniversidad de Concepción
CitationGayana 77(1): 53-59 (2013)
AbstractThis work studied the effect of the lectin phytohaemagglutinin (PHA) and bacterial lipopolysaccharide (LPS) on the activity of nonspecific cytotoxic cells (NCCs) and macrophages of peripheral blood in the rainbow trout. NCCs are cells of the innate immunity, homologues to Natural Killer (NK) cells of mammals, capable of eliminating cells infected with intracellular pathogens and also tumoral cells. Their lytic activity is mediated by the perforin/granzyme system and the Fas receptor-Fas ligand (FasR-FasL) system. Activity of NCCs was evaluated by their capacity to lyse HL-60 tumor cells. On the other hand, macrophages eliminate microorganisms by several mechanisms, including the production of reactive oxygen species (ROS) and nitric oxide (NO). Results showed that PHA significantly increased NCCs cytotoxic activity against HL-60 cells, even under conditions in which the perforins/granzymes lytic system should be suppressed, supporting the existence of the FasR-FasL system in trouts. PHA showed no effect on ROS and NO production by macrophages. Finally, LPS showed no effect on the cytotoxic activity of NCCs or in the production of ROS and NO by macrophages.
URIhttp://hdl.handle.net/10261/95531
DOI10.4067/S0717-65382013000100007
Identifiersdoi: 10.4067/S0717-65382013000100007
issn: 0717-652X
Appears in Collections:(IATS) Artículos
Files in This Item:
File Description SizeFormat 
effect_activity_nonspecific.pdf409,37 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.