Temperature Driven Changeover in the Electron Transfer Mechanism of a Thermophilic Plastocyanin

Abstract

Temperature-Driven Changeover in the Electron-Transfer Mechanism of a Thermophilic Plastocyanin ... thermophilic plastocyanin; protein electron transfer; kinetic regime changeover; 1,ω-alkanedithiol monolayer; temperature; solution viscosity ... For redox proteins immobilized on thiol monolayers (HS–(CH2)n–X), the mechanism transition has often been reported to take place for a protein–electrode distance corresponding to n ≈ 10.(12, 15, 24, 25, 31, 33, 39-43) Moreover, each kinetic regime can be further characterized by a distinct dependence of the ET rate constant on the temperature and solution viscosity,(25-33, 44, 45) though temperature- or viscosity-driven mechanism transitions have not yet been reported for metalloproteins